Fig. 5

Effects of the P2X7R variants on cell-specific functions: cytokine release from PSCs (a–d) and migration of PANC-1 cells (e). a–d IL-6, IL-1β, IL-8 and TNF-α released in the supernatant of PSCs expressing WT (blue), Gly150Arg (orange) and Arg276His (magenta) receptors. Stimulation with BzATP 100 µM and inhibition with AZ10606120 (AZ) 10 µM and A438079 (A43) 10 µM are compared to the respective unstimulated controls cells (CTR). The data are shown as the mean ± SEM for IL-6, IL-1β and TNF-α, and median ± interquartile range for IL-8, of n = 9. e Migration of P2X7R + GFP PANC-1 expressing P2X7R variants. Significance has been evaluated as followed: IL-6, IL-1β and TNF-α statistics have been performed with one sample t test, and reported as *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; IL-8 statistics have been performed with one-sample Wilcoxon signed rank test and reported as *p < 0.05; **p < 0.01.  Migration has been analyzed with the non-parametric Mann–Whitney test and the p values are reported in the graph