Brain-derived neurotrophic factor expression predicts adverse pathological & clinical outcomes in human breast cancer
© Patani et al; licensee BioMed Central Ltd. 2011
Received: 22 February 2011
Accepted: 18 July 2011
Published: 18 July 2011
Brain-derived neurotrophic factor (BDNF) has established physiological roles in the development and function of the vertebrate nervous system. BDNF has also been implicated in several human malignancies, including breast cancer (BC). However, the precise biological role of BDNF and its utility as a novel biomarker have yet to be determined. The objective of this study was to determine the mRNA and protein expression of BDNF in a cohort of women with BC. Expression levels were compared with normal background tissues and evaluated against established pathological parameters and clinical outcome over a 10 year follow-up period.
BC tissues (n = 127) and normal tissues (n = 33) underwent RNA extraction and reverse transcription, BDNF transcript levels were determined using real-time quantitative PCR. BDNF protein expression in mammary tissues was assessed with standard immuno-histochemical methodology. Expression levels were analyzed against tumour size, grade, nodal involvement, TNM stage, Nottingham Prognostic Index (NPI) and clinical outcome over a 10 year follow-up period.
Immuno-histochemical staining revealed substantially greater BDNF expression within neoplastic cells, compared to normal mammary epithelial cells. Significantly higher mRNA transcript levels were found in the BC specimens compared to background tissues (p = 0.007). The expression of BDNF mRNA was demonstrated to increase with increasing NPI; NPI-1 vs. NPI-2 (p = 0.009). Increased BDNF transcript levels were found to be significantly associated with nodal positivity (p = 0.047). Compared to patients who remained disease free, higher BDNF expression was significantly associated with local recurrence (LR) (p = 0.0014), death from BC (p = 0.018) and poor prognosis overall (p = 0.013). After a median follow up of 10 years, higher BDNF expression levels were significantly associated with reduced overall survival (OS) (106 vs. 136 months, p = 0.006). BDNF emerged as an independent prognostic variable in multivariate analysis for disease free survival (DFS) (p = 0.026) and approached significance for OS (p = 0.055).
BDNF expression was found to be significantly higher in BC specimens compared to normal tissue. Higher transcript levels were significantly associated with unfavourable pathological parameters including nodal positivity and increasing NPI; and adverse clinical outcomes including LR, death from BC, poor prognosis, reduced DFS and OS. BDNF offers utility as a prognostic marker and potential for targeted therapeutic strategies.
Introduction & Background
Brain-derived neurotrophic factor (BDNF) belongs to the neurotrophin (NT) superfamily of polypeptide growth factors, which includes nerve growth factor (NGF) and NTs 3-6 [1, 2]. NTs and their receptors have key physiological roles in the development and function of the central and peripheral nervous systems in vertebrates [3–5]. However, they are also widely expressed in non-neuronal tissues . Expression of the BDNF gene (BDNF) is regulated by the presence of multiple activity dependent and tissue-specific promoters . BDNF signals preferentially via its high affinity tyrosine kinase receptor, tropomyosin receptor kinase B (TrkB). Ligand-induced receptor dimerisation results in auto-phosphorylation and initiates multiple signalling cascades, including the mitogen-activated protein kinase (MAPK), phosphatidyl-inositide 3-kinase (PI3K) and phospholipase C-gamma (PLC- γ) pathways, that promote cellular survival [8–11]. However, BDNF also shares a common low affinity receptor (p75NTR) with the other NTs, which is a member of the tumor necrosis factor (TNF) receptor superfamily, implicated in the modulation of cell survival, cell cycle regulation and cytoskeletal rearrangement . The overall cellular response to BDNF exposure is therefore likely to reflect an equilibrium between TrkB and p75NTR activity.
BDNF has been implicated in various human pathologies, including: depression, epilepsy, Alzheimer's, Parkinson's and Huntington's disease [7, 12]. BDNF has also been associated with several human cancers, both neuronal and non-neuronal, including: neuroblastoma , myeloma , ovarian [15, 16], lung , prostate , hepato-cellular , pancreatic [21, 22], head and neck squamous cell carcinomas  and pulmonary carcinoid tumours . Interestingly, the archetypal neurotrophic factor NGF has been demonstrated to stimulate proliferation, angiogenesis and behave as an anti-apoptotic factor in human breast cancer (BC) [1, 25–27] with potential for therapeutic targeting . In keeping with this, BDNF has been associated with cell survival in human BC cell lines . BDNF has also been demonstrated to be significantly up regulated in oestrogen receptor alpha (ER-α) positive BCs . Although increased NT and cognate receptor expression have been demonstrated in BC [2, 30], the precise biological role of BDNF and its utility as a novel biomarker have yet to be determined. The objective of this study was to determine the mRNA and protein expression of BDNF in a cohort of women with BC. Expression levels were compared with normal background tissues and evaluated against established pathological parameters and clinical outcome over a 10 year follow-up period.
Clinical and pathological data
Alive with metastasis
With local recurrence
Died from breast cancer
Died of unrelated disease
ER α negative
ER α positive
ER ß negative
ER ß positive
Tissue Processing, RNA Extraction, cDNA Synthesis & RT-PCR
Forward and reverse primers
Quantitative Analysis of BDNF
BDNF transcript levels within the above-prepared cDNA were determined using real-time quantitative PCR, based on the Amplifluor™ technology, modified from previous reports [31, 32]. Pairs of PCR primers were designed using the Beacon Designer™ software (Version 2, Palo Alto, California, USA) and synthesized by Sigma-Aldrich, added to the reverse primer was an additional sequence, known as the Z sequence (5'-ACTGAACCTGACCGTACA-'3) which is complementary to the universal Z probe (Intergen Inc., Oxford, England, UK). The product expands one intron (Table 2). Taqman detection kit for ß-actin was purchased from Perkin-Elmer. The reaction was carried out using the following: custom made hot-start Q-master mix Abgene (Surrey, England, UK), 10 pmol of specific forward primer, 1 pmol reverse primer with the Z sequence (Table 2), 10 pmol of FAM- (fluorogenic reporter dye, carboxyfluorescein) tagged probe (Intergen Inc.), and cDNA generated from 50 ng RNA. The reaction was carried out using IcyclerIQ™ (Bio-Rad, Hemel Hempstead, England, UK) which is equipped with an optical unit that allows real-time detection of 96 reactions, under the following conditions: 94°C for 12 minutes, 50 cycles of 94°C for 15 seconds, 55°C for 40 seconds and 72°C for 20 seconds. The transcript levels were generated from an internal standard that was simultaneously amplified with the samples. The levels of gene expression were then normalized against the housekeeping control CK-19, which was also quantified in these specimens, to correct for varying amounts of epithelial tissue between samples . With every PCR run, a negative control without a template and a known cDNA reference sample as a positive control, were included.
Immuno-histochemical Analysis of BDNF in Tissues
Frozen sections of breast tissues (normal and tumour) were cut at a thickness of 6µm using a cryostat. Sections were mounted on super frost plus microscope slides, air dried and then fixed in a mixture of 50% Acetone and 50% methanol. Sections were then placed in Optimax wash buffer (Biogenex) for 5-10 minutes to rehydrate. Sections were incubated for 20 minutes in a 10% horse serum blocking solution and then probed with the primary antibody, anti-human BDNF (H-117, SC-20981, Santa Cruz Biotechnologies, Inc.), dilution 1:150 in Optimax buffer (Biogenex). Sections were rinsed thoroughly with wash buffer, before incubation for 30 minutes in the secondary biotinylated antibody (Multi-link Swine anti-goat/mouse/rabbit immunoglobulin, Dako Inc.). Following thorough washings, sections were incubated for 30 minutes with Avidin Biotin Complex (Vector Laboratories) followed by further washings. Di-amino-benzidine (DAB) chromogen (Vector Labs) was then added to the sections which were incubated in the dark for 5 minutes. Sections were then washed for 10 minutes in running tap water, prior to nuclear counter staining with Gill's Haematoxylin for 1 minute, followed by a further 10 minute wash with tap water. Sections were dehydrated in ascending grades of methanol before clearing in xylene and mounting under a cover slip using clear mounting medium.
The two-sample t-test (comparison of mean copy number) was used for statistical analysis of absolute and normalised gene copy number. For normality the Anderson-Darling test was used. The transcript levels within the BC specimens were compared to normal background tissues and analyzed against conventional pathological parameters and clinical outcome over a 10 year follow-up period. In each case the true copy number was used for statistical analysis and hence the samples were not classified as positive or negative. The statistical analysis was carried out using Minitab version 14.1 (Minitab Ltd. Coventry, England, U.K.) using a custom written macro (Stat 2005.mtw). For purposes of the Kaplan-Meier survival analysis, the samples were divided arbitrarily into two groups, 'high transcript level' or 'low transcript level'. The cut-off was guided by the Nottingham Prognostic Index (NPI) value with which the value of the moderate prognostic group was used as the dividing line at the start of the test. NPI = tumour size (cm) × 0.2 + lymph node stage (1 - no nodes affected; 2 - up to 3 nodes affected; 3 - more than 3 nodes affected) + Grade (1-3, Scarff-Bloom-Richardson). NPI scores were classified into three groups: <3.4 = NPI-1, 3.4-5.4 = NPI-2, >5.4 = NPI-3. Survival analysis was performed using SPSS version 16.0 (SPSS Inc. Chicago, IL, USA).
Multivariate analysis of prognostic parameters
Overall Survival (p value)
Disease Free Survival (p value)
NTs and their receptors are increasingly being implicated as novel mediators of carcinogenesis in neuronal and non-neuronal tissues. Whilst the literature regarding BC remains sparse, altered expression and function of these factors are likely to contribute to tumourigenesis and progression. The present study adds to the literature in support of the oncogenic function of BDNF in BC. Furthermore, this study is the first to quantitatively evaluate BDNF mRNA expression in a large cohort of BC patients and provide correlation with conventional pathological parameters and clinical outcomes over an extended follow-up period. Significantly higher mRNA transcript levels were found in BC specimens compared to normal tissue, corroborated at the protein level by immuno-histochemical staining. BDNF expression was found to increase with increasing NPI, nodal positivity, LR, death from BC and poor prognosis overall. After a median follow up of 10 years, higher BDNF expression levels were significantly associated with reduced OS. Furthermore, BDNF remained an independent prognostic variable in multivariate analysis. Our results differ from those of Blasco-Gutierrez et al.  who did not identify differential staining between tumour and normal breast tissue and reported no association between BDNF expression and pathological parameters or clinical outcomes. In their study, Tozlu et al.  employed real-time quantitative reverse transcription (RT)-PCR to compare the mRNA expression of 560 selected genes in BCs excised from 48 women. BDNF emerged as a growth factor significantly up regulated amongst ER-α positive tumours in both the screening and validation sets. However, the relationship was found to be reversed in vitro, where BDNF was found to be up regulated in ER-α negative BC cell lines. Interestingly cross-talk between steroid and growth factor pathways has been identified in the brain, with oestrogen implicated in the regulation of BDNF and demonstrating some degree of overlap in activity with NTs . BDNF has also been associated with increased cell survival, although not proliferation, in human BC cell lines. In accordance with the fact that BDNF did not demonstrate a mitogenic effect, no expression of TrkB was found in any of the BC cells tested. However, the interaction between BDNF and P75NTR, which specifically induces NF-ϰB, has been implicated in the protection of BC cells from apoptosis . Further support for the oncogenic function of BDNF in vivo comes from a transgenic mouse model of spontaneous mammary tumor formation, following exposure to the organochlorine pesticide Dieldrin, which is a persistent environmental toxin thought to increase the risk of BC . Offspring of these mice show enhanced development of mammary tumours with increased mRNA and protein expression of BDNF and TrkB .
The factors regulating NT and NT receptor expression in normal and malignant breast tissues remain unknown. Whilst the precise biological role of the BDNF/TrkB/P75NTR axis in human BC has yet to be elucidated, supporting evidence of oncogenic function may be inferred from other malignancies. TrkB has been implicated in the pathogenesis of neuroblastoma where expression is correlated with poor outcome and a chemo-resistant phenotype [37, 38], increased mortality in Wilm's tumor , lymph node metastasis and advanced stage in non-small cell lung cancer , shorter survival in ovarian cancer  and distant metastases and poor prognosis in gastric cancer . TrkB has also been found to regulate migration, invasion and epithelial-mesenchymal transition in head and neck squamous cell carcinoma . Signalling via the BDNF/TrkB pathway stimulates pro-survival signals, resistance to anoikis and altered cellular aggregation, all features of cancer cells and prerequisites of metastases formation . Emerging roles for NTs in angiogenesis provide further insight into their potential relevance to cancer development and progression . Studies have identified BDNF and TrkB as key mediators of vascular development . BDNF is an endothelial survival factor, deficiency of which results in reduced endothelial cell-cell contacts and apoptosis . BDNF has been implicated as a novel angiogenic protein in multiple myeloma [14, 46]. In support of this, BDNF activation of TrkB has been found to induce vascular endothelial growth factor (VEGF) expression via hypoxia inducible factor-1-alpha (HIF-1-α) in neuroblastoma cells . It has also been suggested that increased expression of TrkB/BDNF may indicate increased neo-neurogenesis, where tumors initiate their own innervation by releasing neurotrophic factors . This may further support tumour progression by the release of neurotransmitters which enhance metastasis. Indeed, the presence of nerve cell markers in tumoral tissue has been found to be a prognostic marker in several human malignancies . In addition to utility as a prognostic marker, therapeutic manipulation of the TrkB signal transduction pathway appears to be an increasingly important target in cancer biology which merits further exploration [11, 50]. Despite the inferences drawn, the mechanisms through which BDNF exerts its oncogenic activity have yet to be adequately determined and this will undoubtedly be necessary to optimise any potential therapeutic applications.
Limitations of the present study include the use of background parenchyma from BC patients to provide 'normal tissue' for comparison is also contentious. Ideally, such material should be derived from patients without BC in order to avoid any 'field change' which may exist within cancer bearing tissues. Although the sample size and follow-up period were substantial, it is possible that a larger cohort, particularly with regard to subgroup analysis, may have influenced several results which approached, but failed to reach, statistical significance. In addition to the measurement of mRNA transcript levels and qualitative immuno-histochemistry, quantitative analysis of protein expression should be undertaken to ensure concordance. Correlation with associated molecules, in particular TrkB and P75NTR, and other markers of invasiveness and metastatic competence would also be of value.
BDNF expression was found to be significantly higher in BC specimens compared to normal tissues. Higher transcript levels were significantly associated with unfavourable pathological parameters including nodal positivity and increasing NPI; and adverse clinical outcomes including LR, death from BC, poor prognosis, reduced DFS and OS. In addition to the prognostic utility of BDNF, further mechanistic studies are warranted to explore the potential for therapeutic manipulation in human BC.
The authors wish to thank Cancer Research Wales and The Fong Family Foundation for supporting this work.
- Descamps S, Toillon RA, Adriaenssens E, Pawlowski V, Cool SM, Nurcombe V, Le Bourhis X, Boilly B, Peyrat JP, Hondermarck H: Nerve growth factor stimulates proliferation and survival of human breast cancer cells through two distinct signaling pathways. J Biol Chem. 2001, 276 (21): 17864-17870. 10.1074/jbc.M010499200.View ArticlePubMedGoogle Scholar
- Blasco-Gutierrez MJ, Jose-Crespo IJ, Zozaya-Alvarez E, Ramos-Sanchez R, Garcia-Atares N: TrkC: a new predictive marker in breast cancer?. Cancer Invest. 2007, 25 (6): 405-410. 10.1080/07357900701206349.View ArticlePubMedGoogle Scholar
- Lewin GR, Barde YA: Physiology of the neurotrophins. Annu Rev Neurosci. 1996, 19: 289-317. 10.1146/annurev.ne.19.030196.001445.View ArticlePubMedGoogle Scholar
- Bibel M, Barde YA: Neurotrophins: key regulators of cell fate and cell shape in the vertebrate nervous system. Genes Dev. 2000, 14 (23): 2919-2937. 10.1101/gad.841400.View ArticlePubMedGoogle Scholar
- Bekinschtein P, Cammarota M, Izquierdo I, Medina JH: BDNF and memory formation and storage. Neuroscientist. 2008, 14 (2): 147-156.View ArticlePubMedGoogle Scholar
- Shibayama E, Koizumi H: Cellular localization of the Trk neurotrophin receptor family in human non-neuronal tissues. Am J Pathol. 1996, 148 (6): 1807-1818.PubMed CentralPubMedGoogle Scholar
- Aid-Pavlidis T, Pavlidis P, Timmusk T: Meta-coexpression conservation analysis of microarray data: a "subset" approach provides insight into brain-derived neurotrophic factor regulation. BMC Genomics. 2009, 10: 420-10.1186/1471-2164-10-420.PubMed CentralView ArticlePubMedGoogle Scholar
- Kaplan DR, Miller FD: Neurotrophin signal transduction in the nervous system. Curr Opin Neurobiol. 2000, 10 (3): 381-391. 10.1016/S0959-4388(00)00092-1.View ArticlePubMedGoogle Scholar
- Huang EJ, Reichardt LF: Trk receptors: roles in neuronal signal transduction. Annu Rev Biochem. 2003, 72: 609-642. 10.1146/annurev.biochem.72.121801.161629.View ArticlePubMedGoogle Scholar
- Miller FD, Kaplan DR: Neurotrophin signalling pathways regulating neuronal apoptosis. Cell Mol Life Sci. 2001, 58 (8): 1045-1053. 10.1007/PL00000919.View ArticlePubMedGoogle Scholar
- Thiele CJ, Li Z, McKee AE: On Trk--the TrkB signal transduction pathway is an increasingly important target in cancer biology. Clin Cancer Res. 2009, 15 (19): 5962-5967. 10.1158/1078-0432.CCR-08-0651.PubMed CentralView ArticlePubMedGoogle Scholar
- Hu Y, Russek SJ: BDNF and the diseased nervous system: a delicate balance between adaptive and pathological processes of gene regulation. J Neurochem. 2008, 105 (1): 1-17. 10.1111/j.1471-4159.2008.05237.x.View ArticlePubMedGoogle Scholar
- Li Z, Tan F, Thiele CJ: Inactivation of glycogen synthase kinase-3beta contributes to brain-derived neutrophic factor/TrkB-induced resistance to chemotherapy in neuroblastoma cells. Mol Cancer Ther. 2007, 6 (12 Pt 1): 3113-3121.View ArticlePubMedGoogle Scholar
- Hu Y, Wang YD, Guo T, Wei WN, Sun CY, Zhang L, Huang J: Identification of brain-derived neurotrophic factor as a novel angiogenic protein in multiple myeloma. Cancer Genet Cytogenet. 2007, 178 (1): 1-10. 10.1016/j.cancergencyto.2007.05.028.View ArticlePubMedGoogle Scholar
- Yu X, Liu L, Cai B, He Y, Wan X: Suppression of anoikis by the neurotrophic receptor TrkB in human ovarian cancer. Cancer Sci. 2008, 99 (3): 543-552. 10.1111/j.1349-7006.2007.00722.x.View ArticlePubMedGoogle Scholar
- Au CW, Siu MK, Liao X, Wong ES, Ngan HY, Tam KF, Chan DC, Chan QK, Cheung AN: Tyrosine kinase B receptor and BDNF expression in ovarian cancers - Effect on cell migration, angiogenesis and clinical outcome. Cancer Lett. 2009, 281 (2): 151-161. 10.1016/j.canlet.2009.02.025.View ArticlePubMedGoogle Scholar
- Ricci A, Greco S, Mariotta S, Felici L, Bronzetti E, Cavazzana A, Cardillo G, Amenta F, Bisetti A, Barbolini G: Neurotrophins and neurotrophin receptors in human lung cancer. Am J Respir Cell Mol Biol. 2001, 25 (4): 439-446.View ArticlePubMedGoogle Scholar
- Bronzetti E, Artico M, Forte F, Pagliarella G, Felici LM, D'Ambrosio A, Vespasiani G, Bronzetti B: A possible role of BDNF in prostate cancer detection. Oncol Rep. 2008, 19 (4): 969-974.PubMedGoogle Scholar
- Yang ZF, Ho DW, Lam CT, Luk JM, Lum CT, Yu WC, Poon RT, Fan ST: Identification of brain-derived neurotrophic factor as a novel functional protein in hepatocellular carcinoma. Cancer Res. 2005, 65 (1): 219-225.PubMedGoogle Scholar
- Schneider MB, Standop J, Ulrich A, Wittel U, Friess H, Andren-Sandberg A, Pour PM: Expression of nerve growth factors in pancreatic neural tissue and pancreatic cancer. J Histochem Cytochem. 2001, 49 (10): 1205-1210. 10.1177/002215540104901002.View ArticlePubMedGoogle Scholar
- Miknyoczki SJ, Wan W, Chang H, Dobrzanski P, Ruggeri BA, Dionne CA, Buchkovich K: The neurotrophin-trk receptor axes are critical for the growth and progression of human prostatic carcinoma and pancreatic ductal adenocarcinoma xenografts in nude mice. Clin Cancer Res. 2002, 8 (6): 1924-1931.PubMedGoogle Scholar
- Sclabas GM, Fujioka S, Schmidt C, Li Z, Frederick WA, Yang W, Yokoi K, Evans DB, Abbruzzese JL, Hess KR: Overexpression of tropomysin-related kinase B in metastatic human pancreatic cancer cells. Clin Cancer Res. 2005, 11 (2 Pt 1): 440-449.PubMedGoogle Scholar
- Kupferman ME, Jiffar T, El-Naggar A, Yilmaz T, Zhou G, Xie T, Feng L, Wang J, Holsinger FC, Yu D: TrkB induces EMT and has a key role in invasion of head and neck squamous cell carcinoma. Oncogene. 29 (14): 2047-2059.Google Scholar
- Ricci A, Graziano P, Mariotta S, Cardillo G, Sposato B, Terzano C, Bronzetti E: Neurotrophin system expression in human pulmonary carcinoid tumors. Growth Factors. 2005, 23 (4): 303-312. 10.1080/08977190500233813.View ArticlePubMedGoogle Scholar
- Descamps S, Lebourhis X, Delehedde M, Boilly B, Hondermarck H: Nerve growth factor is mitogenic for cancerous but not normal human breast epithelial cells. J Biol Chem. 1998, 273 (27): 16659-16662. 10.1074/jbc.273.27.16659.View ArticlePubMedGoogle Scholar
- Romon R, Adriaenssens E, Lagadec C, Germain E, Hondermarck H, Le Bourhis X: Nerve growth factor promotes breast cancer angiogenesis by activating multiple pathways. Mol Cancer. 9: 157-Google Scholar
- Dolle L, Adriaenssens E, El Yazidi-Belkoura I, Le Bourhis X, Nurcombe V, Hondermarck H: Nerve growth factor receptors and signaling in breast cancer. Curr Cancer Drug Targets. 2004, 4 (6): 463-470. 10.2174/1568009043332853.View ArticlePubMedGoogle Scholar
- Adriaenssens E, Vanhecke E, Saule P, Mougel A, Page A, Romon R, Nurcombe V, Le Bourhis X, Hondermarck H: Nerve growth factor is a potential therapeutic target in breast cancer. Cancer Res. 2008, 68 (2): 346-351. 10.1158/0008-5472.CAN-07-1183.View ArticlePubMedGoogle Scholar
- Tozlu S, Girault I, Vacher S, Vendrell J, Andrieu C, Spyratos F, Cohen P, Lidereau R, Bieche I: Identification of novel genes that co-cluster with estrogen receptor alpha in breast tumor biopsy specimens, using a large-scale real-time reverse transcription-PCR approach. Endocr Relat Cancer. 2006, 13 (4): 1109-1120. 10.1677/erc.1.01120.View ArticlePubMedGoogle Scholar
- Popnikolov NK, Cavone SM, Schultz PM, Garcia FU: Diagnostic utility of p75 neurotrophin receptor (p75NTR) as a marker of breast myoepithelial cells. Mod Pathol. 2005, 18 (12): 1535-1541.PubMedGoogle Scholar
- Jiang WG, Watkins G, Lane J, Cunnick GH, Douglas-Jones A, Mokbel K, Mansel RE: Prognostic value of rho GTPases and rho guanine nucleotide dissociation inhibitors in human breast cancers. Clin Cancer Res. 2003, 9 (17): 6432-6440.PubMedGoogle Scholar
- Jiang WG, Douglas-Jones A, Mansel RE: Expression of peroxisome-proliferator activated receptor-gamma (PPARgamma) and the PPARgamma co-activator, PGC-1, in human breast cancer correlates with clinical outcomes. Int J Cancer. 2003, 106 (5): 752-757. 10.1002/ijc.11302.View ArticlePubMedGoogle Scholar
- Jiang WG, Watkins G, Fodstad O, Douglas-Jones A, Mokbel K, Mansel RE: Differential expression of the CCN family members Cyr61, CTGF and Nov in human breast cancer. Endocr Relat Cancer. 2004, 11 (4): 781-791. 10.1677/erc.1.00825.View ArticlePubMedGoogle Scholar
- Sohrabji F, Lewis DK: Estrogen-BDNF interactions: implications for neurodegenerative diseases. Front Neuroendocrinol. 2006, 27 (4): 404-414. 10.1016/j.yfrne.2006.09.003.PubMed CentralView ArticlePubMedGoogle Scholar
- Hoyer AP, Grandjean P, Jorgensen T, Brock JW, Hartvig HB: Organochlorine exposure and risk of breast cancer. Lancet. 1998, 352 (9143): 1816-1820. 10.1016/S0140-6736(98)04504-8.View ArticlePubMedGoogle Scholar
- Cameron HL, Foster WG: Developmental and lactational exposure to dieldrin alters mammary tumorigenesis in Her2/neu transgenic mice. PLoS One. 2009, 4 (1): e4303-10.1371/journal.pone.0004303.PubMed CentralView ArticlePubMedGoogle Scholar
- Jaboin J, Kim CJ, Kaplan DR, Thiele CJ: Brain-derived neurotrophic factor activation of TrkB protects neuroblastoma cells from chemotherapy-induced apoptosis via phosphatidylinositol 3'-kinase pathway. Cancer Res. 2002, 62 (22): 6756-6763.PubMedGoogle Scholar
- Aoyama M, Asai K, Shishikura T, Kawamoto T, Miyachi T, Yokoi T, Togari H, Wada Y, Kato T, Nakagawara A: Human neuroblastomas with unfavorable biologies express high levels of brain-derived neurotrophic factor mRNA and a variety of its variants. Cancer Lett. 2001, 164 (1): 51-60. 10.1016/S0304-3835(00)00715-1.View ArticlePubMedGoogle Scholar
- Eggert A, Grotzer MA, Ikegaki N, Zhao H, Cnaan A, Brodeur GM, Evans AE: Expression of the neurotrophin receptor TrkB is associated with unfavorable outcome in Wilms' tumor. J Clin Oncol. 2001, 19 (3): 689-696.PubMedGoogle Scholar
- Zhang S, Guo D, Luo W, Zhang Q, Zhang Y, Li C, Lu Y, Cui Z, Qiu X: TrkB is highly expressed in NSCLC and mediates BDNF-induced the activation of Pyk2 signaling and the invasion of A549 cells. BMC Cancer. 10: 43-Google Scholar
- Zhang Y, Fujiwara Y, Doki Y, Takiguchi S, Yasuda T, Miyata H, Yamazaki M, Ngan CY, Yamamoto H, Ma Q: Overexpression of tyrosine kinase B protein as a predictor for distant metastases and prognosis in gastric carcinoma. Oncology. 2008, 75 (1-2): 17-26. 10.1159/000151615.View ArticlePubMedGoogle Scholar
- Douma S, Van Laar T, Zevenhoven J, Meuwissen R, Van Garderen E, Peeper DS: Suppression of anoikis and induction of metastasis by the neurotrophic receptor TrkB. Nature. 2004, 430 (7003): 1034-1039. 10.1038/nature02765.View ArticlePubMedGoogle Scholar
- Kermani P, Rafii D, Jin DK, Whitlock P, Schaffer W, Chiang A, Vincent L, Friedrich M, Shido K, Hackett NR: Neurotrophins promote revascularization by local recruitment of TrkB+ endothelial cells and systemic mobilization of hematopoietic progenitors. J Clin Invest. 2005, 115 (3): 653-663.PubMed CentralView ArticlePubMedGoogle Scholar
- Kim H, Li Q, Hempstead BL, Madri JA: Paracrine and autocrine functions of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in brain-derived endothelial cells. J Biol Chem. 2004, 279 (32): 33538-33546. 10.1074/jbc.M404115200.View ArticlePubMedGoogle Scholar
- Donovan MJ, Lin MI, Wiegn P, Ringstedt T, Kraemer R, Hahn R, Wang S, Ibanez CF, Rafii S, Hempstead BL: Brain derived neurotrophic factor is an endothelial cell survival factor required for intramyocardial vessel stabilization. Development. 2000, 127 (21): 4531-4540.PubMedGoogle Scholar
- Hu Y, Sun CY, Wang HF, Guo T, Wei WN, Wang YD, He WJ, Wu T, Tan H, Wu TC: Brain-derived neurotrophic factor promotes growth and migration of multiple myeloma cells. Cancer Genet Cytogenet. 2006, 169 (1): 12-20. 10.1016/j.cancergencyto.2006.02.018.View ArticlePubMedGoogle Scholar
- Nakamura K, Martin KC, Jackson JK, Beppu K, Woo CW, Thiele CJ: Brain-derived neurotrophic factor activation of TrkB induces vascular endothelial growth factor expression via hypoxia-inducible factor-1alpha in neuroblastoma cells. Cancer Res. 2006, 66 (8): 4249-4255. 10.1158/0008-5472.CAN-05-2789.View ArticlePubMedGoogle Scholar
- Entschladen F, Palm D, Niggemann B, Zaenker KS: The cancer's nervous tooth: Considering the neuronal crosstalk within tumors. Semin Cancer Biol. 2008, 18 (3): 171-175. 10.1016/j.semcancer.2007.12.004.View ArticlePubMedGoogle Scholar
- Entschladen F, Palm D, Lang K, Drell TLt, Zaenker KS: Neoneurogenesis: tumors may initiate their own innervation by the release of neurotrophic factors in analogy to lymphangiogenesis and neoangiogenesis. Med Hypotheses. 2006, 67 (1): 33-35. 10.1016/j.mehy.2006.01.015.View ArticlePubMedGoogle Scholar
- Desmet CJ, Peeper DS: The neurotrophic receptor TrkB: a drug target in anti-cancer therapy?. Cell Mol Life Sci. 2006, 63 (7-8): 755-759. 10.1007/s00018-005-5490-8.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.