- Primary research
- Open Access
Silencing SATB1 with siRNA inhibits the proliferation and invasion of small cell lung cancer cells
© Huang et al; licensee BioMed Central Ltd. 2013
Received: 15 November 2012
Accepted: 30 January 2013
Published: 5 February 2013
Small cell lung cancer (SCLC) is a special kind of lung cancers, lymph or blood metastasis of SCLC usually occurs in early stage. Studies in breast and colon cancer showed over expression of SATB1 could promote tumor cell growth and inhibit apoptosis. Therefore, we studied the expression of SATB1 in SCLC.
The level of SATB1 was analyzed in SCLC tissues, metastatic lymphoid nodes and adjacent normal lung tissues by immunohistochemistry. Meanwhile, small interfering SATB1-targeting RNA was constructed and transfected into human SCLC cell line NCI-H446 to evaluate the effects of SATB1-siRNA on cell proliferation, invasion and apoptosis.
SATB1 protein was overexpressed in SCLC tissues and metastasis lymphoid nodes compared with adjacent normal lung tissues. Compared with control group, SATB1-siRNA inhibits the proliferation and invasion of SCLC cells and induces SCLC cells apoptosis statistically (P<0.05) in vitro.
Our results suggest that SATB1 plays an important role in the metastasis of human SCLC cell.
Small cell lung cancer (SCLC) is a special kind of lung cancer, which comprises 15%-20% of all lung cancers. Different from other lung cancers, SCLC exhibits aggressive behavior with rapid growth and spread to distant sites early. Although SCLC is exquisite sensitivity to chemotherapy and radiation, it is more prone to relapse and recurrence. Special AT-rich sequence binding protein 1 (SATB1) is a nuclear matrix attachment region binding protein (MBP) which participates in higher-order chromatin organization and tissue-specific gene expression[2, 3]. SATB1 play an important role in differentiation, and expresses at high level in thymocyte, its expression is down-regulated in mature T cells[4, 5]. SATB1 has been reported to promote a metastatic phenotype and correlate with poor prognosis in breast cancer. Its expression has also been associated with unfavourable clinicopathological characteristics and poor prognosis in gastric, liver and colorectal cancer and glioma[7–12]. RNA-interference-mediated knockdown in aggressive cancer cells have altered the expression of over 1,000 genes, this technique effectively inhibited cell capacity of proliferation, invasion, tumor growth and metastasis. Zheng et al. also found SATB1 expression in aggressive rather than non-aggressive breast cancer cells. Similarly, transduction of SATB1into non-metastatic cells led to the invasion of tumors in mice, whereas silencing SATB1 lead cells to their normal phenotype and prevented tumor growth and metastasis. RNA interference (RNAi) targeting SATB1 has been successfully used in sarcoma, leukemia and pancreatic cells, few report has been published concerning the effect of small interfering RNA (siRNA) on the SATB1 gene in SCLC cells, in our study, we investigated the expression of SATB 1 and its role in the pathogenesis of SCLC by knocking down SATB1 in NCI-H446 cells.
Materials and methods
SCLC specimens were obtained from 29 cases of SCLC patients underwent surgery in the First Affiliated Hospital of Liaoning Medical University from January 2009 to December 2012. Neither chemotherapy nor radiotherapy was conducted in these patients. Acquisition and analysis of these operation sample was approved by the ethics committee of hospital, the patient also signed the informed consent.
Cell line and cell culture
Human SCLC cell line (NCI-H446) (Shanghai Biological Sciences Institute, China) was used to analysis the expression of SATB 1 protein interfered by siRNA. NCI-H446 cells were cultured in RPMI1640 supplemented with 10% fetal bovine serum (FBS; Hyclone), 10 units/L penicillin G and 100 mg/L streptomycin at 37°C in a humidified atmosphere containing 5% CO2.
Immunohistochemical analysis was conducted to observe the expression of SATB 1 in SCLC tissues, metastasis lymphoid node tissue and lung cancer adjacent tissue. Briefly, tissues were fixed with 4% paraformaldehyde and embedded with paraffin using standard methods. IHC was carried out using a specific rabbit polyclonal anti-SATB1 antibody (1:250; Jingmei Biotech Corp, Beijing, China) according to the provided instruction of supplier. The brown staining in cytoplasm was defined as the positive signal. The scoring criteria were used as reported previously. Briefly, the staining proportion was classified as 1 (<10%), 2 (10–25%), 3 (26–75%), and 4 (>75%). The staining intensity was graded as 0 for negative, 1 for weak, 2 for moderate, and 3 for strong staining. The staining index was subsequently obtained by multiplication of the proportion and intensity and calculated index was finally assessed by a simplified score (score 0, index 0–1; score 1, index 2–4; score 2, index 6–8; score 3, index 9–12). Samples with staining score of at least one were classified as the positive staining.
Based on the SATB1 cDNA sequence in Gene Bank, 3 pairs of synthesized oligonucleotide were designed (Dalian Biotechnologies, Dalian, China). The sequences used were as follows: (Si-1) F: 5′- GAT CCC CGG ATT TGG AAG AGA GTG TCT TCA AGA GAG ACA CTC TCT TCC AAA TCC TTT TTG GAA A-3′; (Si-1) R: 5′- AGC TTT TCC AAA AAG GAT TTG GAA GAG AGT GTC TCT CTT GAA GAC ACT CTC TTC CAA ATC CGG G -3′; (Si-2) F: 5′- GAT CCC CGT CCA CCT TGT CTT CTC TCT TCA AGA GAG AGA GAA GAC AAG GTG GAC TTT TTG GAA A -3′ (Si-2) R: 5′- AGC TTT TCC AAA AAG TCC ACC TTG TCT TCT CTC TCT CTT GAA GAG AGA AGA CAA GGT GGA CGG G- 3′; (Si-N) (control group) F: 5′-GAT CCG CGA GAC CTC AGT ATG TTA CCT GTG AAG CCA CAG ATG GGG TAAC ATA CTG AGG TCT CGC TTT TTT G -3′. Oligonucleotide was annealed and ligated with pRNAT- U6.1/ Neo-siRNA using T4 DNA ligase. The three constructed recombinant plasmids- SATB1-siRNA-1, SATB1-siRNA-2, and SATB1-siRNA-N were verified by sequencing and restriction endonuclease-digestion.
NCI-H446 cells were seeded (2 × 105 cells / well) in six-well plates. After incubation for 24 hours, they were transfected by SATB1-siRNA-1, SATB1-siRNA-2, and SATB1-siRNA-N plasmid in serum-free medium by Lipofectamine 2000 regent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instruction.
Western blot analysis SATB 1 expression level
Cells were washed twice with ice-cold PBS and then treated with lysis buffer for 30 minutes at 4°C. The supernatants were centrifuged at 12000 g 30 minutes at 4°C. Protein samples were electrophoresed on 7.5% SDS-PAGE gels and transferred to PVDF membrane. Nonspecific reactivity was blocked in 5% nonfat dry milk in TBST for 1 hour at room temperature. The membrane was then treated with rabbit anti-human SATB1 antibody (1:250; Jingmei Biotech Corp, Beijing, China) overnight at 4°C, followed by reaction to goat anti-rabbit antibody (1:500; Jingmei Biotech Corp, Beijing, China). Each sample was also probed with β-actin antibody (Sigma-Aldrich Corp.) as a loading control. The intensity of the detected bands was analyzed using Image J program.
Cell proliferation assay
Cells were treated with siRNA as described above for 48 hours. Then cells (2 × 103 per well) were plated in 96-well plates cultured from 24 to 96 hours, MTT assay was performed to analysis growth of these cells.
SCLC cells invasion assays
The invasion of SCLC cells was conducted using Matrigel coated 24-well trans-well chamber (Costar). 48 h later, serum-free RPMI-1640 containing 2.0 × 105 cells was introduced into the upper compartment and the lower compartment contained RPMI-1640 supplemented with 15% FCS. Incubated at 37°C for 24 h, the non-invasive cells from the upper surface of the membrane were removed completely with cotton swabs. The invasive cells attached to the lower membrane surface were fixed with 4% paraformaldehyde and stained with hematoxylin and eosin (HE). The number of invasive cells was counted under microscope (200X).
After 48 hours, cells were isolated and stained with Propidium Iodide (PI) (BD Biosciences Clontech, Palo Alto, CA) 48 hours after interfered. They were analyzed using FACS according fluorescence of PI. The fraction of apoptotic cells in the siRNA treated population was determined by the super-enhanced DMax method of WinList software (Verity Software House, Topsham, ME).
SPSS 17.0 software was used in statistical analysis. One-way ANOVA was performed for multiple comparisons. The frequencies of MEKK1 expression among cancer samples were analyzed by the test with modification by the Fisher’s exact test to account for frequency values < 5. The differences were considered significant at p < 0.05.
Expressions of SATB1 in 29 SCLC patients
Clinical characteristic of patients to the expression of MEKK1
Patient number (n)
Staining score of SATB1
SATB1-siRNA inhibited SATB1 expression in lung cancer cells
SATB1-siRNA induced morphology changes
SATB1-siRNA inhibited NCI-H446 cell proliferation and induced NCI-H446 cells apoptosis
SATB1-siRNA inhibited NCI-H446 cell invasion
SCLC, of which lymph nodes metastasis usually occurs in early stage, is a special kind of lung cancers. Although radiotherapy and chemotherapy have produced modest benefits in some patients, it would relapse and resistant to many drugs after traditional therapies. This is particularly important for early detection and providing reliable therapeutic target.
In this study, we verified SATB1 was overexpressed in the SCLC tissues and metastatic lymph nodes tissues, small interfering RNA targeting SATB1- SATB1- siRNA-1 and SATB1-siRNA-2 were constructed successfully, our results showed that transfection with SATB1- siRNA-1 or SATB1-siRNA-2 into the SCLC cell-NCI-H446, could inhibit the cells proliferation and invasion significantly. In addition, SATB1-SiRNA could induce the apoptosis of SCLC cells in vitro.
SATB1 was originally characterized as a regulator in T cell differentiation, found to be overexpressed in metastatic breast cancer cell lines and in human tissue specimens from advanced stages of breast carcinomas with metastasis[2, 4, 19–21]. There are a few studies reported the expression of SATB1 in lung cancers, but the role of SATB1 is controversial: a study in squamous cell lung cancer and non-small cell lung cancers (NSCLC) showed that SATB1 expression was lost and the loss of SATB1 predicted poor prognosis in squamous cell carcinomas, Zhou et al. showed the expression of SATB1 mRNA was much higher in NSCLC tissues with or without metastasis than in normal lung tissues. In our study, we found SATB1 was highly expressed in SCLC tissues and metastatic lymphoid nodes tissues compared with lung cancer adjacent tissue, SATB1-siRNA could effectively inhibit SATB1 expression in SCLC cells. Treatment of SCLC cell lines with SATB1-siRNA resulted in morphologic changes in these cells. Our result also showed SATB1-siRNA could induce SCLC cells apoptosis after transfecting SATB1-SiRNA into SCLC cells. These results suggest SATB1 might be an ideal target for the treatment of SCLC. Metastasis is the final step in solid tumor progression and is the most common cause of death in cancer patients. Controlling the invasion and migration can improve the survival rate of cancer patients. Recent study demonstrated that the SATB1 plays an important role in the process of invasion and migration. Consistent with the previous study, our results also showed the invasion ability of SCLC cells declined obviously after transfected with SATB1-SiRNA.
About the mechanisms why SATB1 influence the proliferation and invasion for small lung cancer cell, so far there is no much research on that, however, lots of study about the roles of SATB1 in breast cancer and other cancers have been investigated, Han et al. use gene chip to find SATB1 can induce the change of more than 1000 genes’ expression. Some are associated with cancer invasion and metastasis, such as MMP2, MMP9, CTGF, et al.. We will further study its detailed mechanism in the small lung cancer cell.
In summary, our work provides a better understanding of the physiological role of SATB1 in SCLC, SATB1 could regulate the invasion and migration of SCLC cells, this may provide important clues for more effective targeting of SCLC and other cancers with aberrant SATB1 activation.
This work was supported by the Educational Department of Liaoning Province (Series number: L2010302, L2010298) and the Science and Technology Department of Liaoning Province (series number: 201202143). The authors declare no conflict of interests.
- Lally BE, Urbanic JJ, Blackstock AW, Miller AA, Perry MC: Small cell lung cancer: have we made any progress over the last 25 years?. Oncologist. 2007, 12 (9): 1096-1104. 10.1634/theoncologist.12-9-1096.View ArticlePubMedGoogle Scholar
- Nakayama Y, Mian IS, Kohwi-Shigematsu T, Ogawa T: A nuclear targeting determinant for SATB1, a genome organizer in the T cell lineage. Cell Cycle. 2005, 4 (8): 1099-1106.View ArticlePubMedGoogle Scholar
- Cai S, Han HJ, Kohwi-Shigematsu T: Tissue-specific nuclear architecture and gene expression regulated by SATB1. Nat Genet. 2003, 34 (1): 42-51. 10.1038/ng1146.View ArticlePubMedGoogle Scholar
- Wen J, Huang S, Rogers H, Dickinson LA, Kohwi-Shigematsu T, Noguchi CT: SATB1 family protein expressed during early erythroid differentiation modifies globin gene expression. Blood. 2005, 105 (8): 3330-3339. 10.1182/blood-2004-08-2988.View ArticlePubMedGoogle Scholar
- Nie H, Yao X, Maika SD, Tucker PW: SATB1 is required for CD8 coreceptor reversal. Mol Immunol. 2008, 46 (1): 207-211. 10.1016/j.molimm.2008.07.007.View ArticlePubMedGoogle Scholar
- Han HJ, Russo J, Kohwi Y, Kohwi-Shigematsu T: SATB1 reprogrammes gene expression to promote breast tumour growth and metastasis. Nature. 2008, 452 (7184): 187-193. 10.1038/nature06781.View ArticlePubMedGoogle Scholar
- Cheng C, Lu X, Wang G, Zheng L, Shu X, Zhu S, Liu K, Wu K, Tong Q: Expression of SATB1 and heparanase in gastric cancer and its relationship to clinicopathologic features. APMIS: acta pathologica, microbiologica, et immunologica Scandinavica. 2010, 118 (11): 855-863. 10.1111/j.1600-0463.2010.02673.x.View ArticlePubMedGoogle Scholar
- Lu X, Cheng C, Zhu S, Yang Y, Zheng L, Wang G, Shu X, Wu K, Liu K, Tong Q: SATB1 is an independent prognostic marker for gastric cancer in a Chinese population. Oncol Rep. 2010, 24 (4): 981-987.PubMedGoogle Scholar
- Tu W, Luo M, Wang Z, Yan W, Xia Y, Deng H, He J, Han P, Tian D: Upregulation of SATB1 promotes tumor growth and metastasis in liver cancer. Liver Int. 2012, 32 (7): 1064-1078. 10.1111/j.1478-3231.2012.02815.x.View ArticlePubMedGoogle Scholar
- Chu SH, Ma YB, Feng DF, Zhang H, Zhu ZA, Li ZQ, Jiang PC: Upregulation of SATB1 is associated with the development and progression of glioma. J Transl Med. 2012, 10: 149-10.1186/1479-5876-10-149.PubMed CentralView ArticlePubMedGoogle Scholar
- Nodin B, Johannesson H, Wangefjord S, DP OC, Ericson-Lindquist K, Uhlen M, Jirstrom K, Eberhard J: Molecular correlates and prognostic significance of SATB1 expression in colorectal cancer. Diagn Pathol. 2012, 7 (1): 115-10.1186/1746-1596-7-115.PubMed CentralView ArticlePubMedGoogle Scholar
- Meng WJ, Yan H, Zhou B, Zhang W, Kong XH, Wang R, Zhan L, Li Y, Zhou ZG, Sun XF: Correlation of SATB1 overexpression with the progression of human rectal cancer. Int J Colorectal Dis. 2012, 27 (2): 143-150. 10.1007/s00384-011-1302-9.View ArticlePubMedGoogle Scholar
- Zheng J: Is SATB1 a master regulator in breast cancer growth and metastasis?. Womens Health (Lond Engl). 2008, 4 (4): 329-332. 10.2217/17455057.4.4.329.View ArticleGoogle Scholar
- Neznanov N, Kohwi-Shigematsu T, Oshima RG: Contrasting effects of the SATB1 core nuclear matrix attachment region and flanking sequences of the keratin 18 gene in transgenic mice. Mol Biol Cell. 1996, 7 (4): 541-552.PubMed CentralView ArticlePubMedGoogle Scholar
- Zimmerman MA, Rahman NT, Yang D, Lahat G, Lazar AJ, Pollock RE, Lev D, Liu K: Unphosphorylated SATB1 promotes sarcoma development through repressing expression of Fas and bad and conferring apoptotic resistance. Cancer Res. 2012, 72 (18): 4724-4732. 10.1158/0008-5472.CAN-12-1347.PubMed CentralView ArticlePubMedGoogle Scholar
- Hubackova S, Krejcikova K, Bartek J, Hodny Z: Interleukin 6 signaling regulates promyelocytic leukemia protein gene expression in human normal and cancer cells. J Biol Chem. 2012, 287 (32): 26702-26714. 10.1074/jbc.M111.316869.PubMed CentralView ArticlePubMedGoogle Scholar
- Moore F, Cunha DA, Mulder H, Eizirik DL: Use of RNA interference to investigate cytokine signal transduction in pancreatic beta cells. Methods Mol Biol. 2012, 820: 179-194. 10.1007/978-1-61779-439-1_11.View ArticlePubMedGoogle Scholar
- Su F, Li H, Yan C, Jia B, Zhang Y, Chen X: Depleting MEKK1 expression inhibits the ability of invasion and migration of human pancreatic cancer cells. J Cancer Res Clin Oncol. 2009, 135 (12): 1655-1663. 10.1007/s00432-009-0612-6.View ArticlePubMedGoogle Scholar
- Hanker LC, Karn T, Mavrova-Risteska L, Ruckhaberle E, Gaetje R, Holtrich U, Kaufmann M, Rody A, Wiegratz I: SATB1 gene expression and breast cancer prognosis. Breast. 2011, 20 (4): 309-313. 10.1016/j.breast.2010.10.002.View ArticlePubMedGoogle Scholar
- Li QQ, Chen ZQ, Xu JD, Cao XX, Chen Q, Liu XP, Xu ZD: Overexpression and involvement of special AT-rich sequence binding protein 1 in multidrug resistance in human breast carcinoma cells. Cancer Sci. 2010, 101 (1): 80-86. 10.1111/j.1349-7006.2009.01372.x.View ArticlePubMedGoogle Scholar
- Patani N, Jiang W, Mansel R, Newbold R, Mokbel K: The mRNA expression of SATB1 and SATB2 in human breast cancer. Cancer Cell Int. 2009, 9: 18-10.1186/1475-2867-9-18.PubMed CentralView ArticlePubMedGoogle Scholar
- Selinger CI, Cooper WA, Al-Sohaily S, Mladenova DN, Pangon L, Kennedy CW, McCaughan BC, Stirzaker C, Kohonen-Corish MR: Loss of special AT-rich binding protein 1 expression is a marker of poor survival in lung cancer. J Thorac Oncol. 2011, 6 (7): 1179-1189. 10.1097/JTO.0b013e31821b4ce0.View ArticlePubMedGoogle Scholar
- Zhou LY LF, Tong J, Chen QQ, Zhang FW: Expression of special AT-rich sequence-binding protein mRNA and its clinicopathological significance in non-small cell lung cancer. Nan fang yi ke da xue xue bao = J South Med Univ. 2009, 29: 534-537.Google Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.