Effect of wine on Akt in H1299 NSCLC cells. Total cell lysates were prepared from H1299 cells that were serum-deprived for 24 h then treated with or without 2% red wine (Pinot Noir) (A) or 5% white wine (Riesling) (B) for 24 h. Cell lysates (25 μg) were resolved by SDS-PAGE and immunoblotted with anti-phospho or anti-total- Akt antibody. Representative immunoblots are shown. Immunoblots were scanned to quantitate the density of the bands. All values are arbitrary densitometry units expressed relative to untreated control.