Elevated expression of phospho-histone H2B S(14) in AR-knockdown LNCaP cells. LNCaP cells were either transfected with pSiAR-EGFP or pSiCon-EGFP plasmid construct. On day 6 after transfection, cells were harvested. Cellular proteins were extracted using acid extraction method, electrophoresized through gradient Tris-HCl gels, and electroblotted onto PVDF membranes. Levels of phospho-histone H2B expression was detected by an immunoassay procedure.