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Fig. 6 | Cancer Cell International

Fig. 6

From: Selected polyphenols potentiate the apoptotic efficacy of glycolytic inhibitors in human acute myeloid leukemia cell lines. Regulation by protein kinase activities

Fig. 6

Effect of 2-deoxy-d-glucose and quercetin on protein kinase activities. The figure shows the relative levels of phosphorylated (P) and total (T) Akt, ERKs, S6 ribosomal protein (rpS6), GSK-3α,β, and p38-MAPK, and β-actin (assessed as a control of sample loading). HL60 cells were kept untreated, incubated for 1 or 6 h with 5 mM 2-DG alone, or incubated for 2 h with 20 μM Quer and then for 1 or 6 h more with or without addition of 2-DG. Whenever possible, band intensities of phosphorylated forms were measured, normalized with to the corresponding total form, and expressed in relation the Cont (arbitrary value of 1.0) (see values within the blots: nd not determined). ERK1/ERK2 were measured together, while GSK-3α and GSK-3β were separately analyzed. The blots are representative of one of at least three independent determinations, with qualitatively similar results. For other conditions see legend of Fig. 1

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Cancer Cell International

ISSN: 1475-2867

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