Fig. 4

Casticin induces G0/G1 arrest by inactiving the AKT pathway. a–c NOZ and SGC996 cells were treated with casticin (0, 1, 4, 7 µM) for 48 h. The cell cycle distribution was analyzed by flow cytometry. d Expression levels of cyclinD1, CDK4, p27, p-AKT and AKT were measured using western blot analysis. e After pretreatment with 50 µM LY294002 (Akt inhibitor) for 1 h, GBC cells were incubated with 4 µM casticin for 24 h, and cellular viability was determined. f Cellular viability was determined after treatment with 4 µM casticin in cells transfected with WT-Akt, CA-Akt, or vehicle plasmid. All data are presented as the means ± standard deviations. Significant differences compared with the control were indicated by *p < 0.05, **p < 0.01, and ***p < 0.001