Fig. 4

miR-4532 suppressed the expression of the HIC-1 gene. a Sequence alignment between miR-4532 and the 3′-UTR of HIC-1. b Effects of miR-4532 on HIC-1 were assessed with the luciferase reporter system. The miR-4532 mimic, together with the luciferase reporter vector or control vector, was transfected into MCF-7 cells (NS: not significant, Student’s t-test). c qPCR was used to measure the expression levels of HIC-1 mRNA in MCF-7 cells and MCF-7 cells transfected with the miR-4532 mimic or negative control (***p < 0.01, Student’s t-test). d Western blot analysis of HIC-1 protein expression in MCF-7 cells and MCF-7 cells transfected with the miR-4532 mimic or negative control