Fig. 5

HIC-1 inhibited breast cancer cell invasion and metastasis in vitro. a In vitro transwell assays for MCF-7^HIC−1 and MCF-7^NC cells with or without IL-6 stimulation. b In vitro transwell assays for MCF-7^sh-HIC−1 and MCF-7^NC cells with or without IL-6 stimulation. c In vitro transwell assay for MCF-7 and MCF-7^sh-HIC−1 cells treated with the STAT3 inhibitor AG490 (100 μM), and western blot analysis of proteins in the STAT3 signaling pathway. The invading cells were observed and analyzed using a microscope with a ×10 objective. Student’s t-tests: NS: p > 0.05, **p < 0.05, ***p < 0.01, SPSS