Fig. 4From: S-Adenosylmethionine regulates apoptosis and autophagy in MCF-7 breast cancer cells through the modulation of specific microRNAsAutophagy occurrence in MCF-7 after AdoMet and miR-486-5p mimic or inhibitor treatment. Cells were not treated (Control) or treated with 500 μM AdoMet and/or transfected with miR486-5p mimic or miR-486-5p inhibitor for 48 and 72 h. MCF-7 cells were exposed to LTR and analyzed by flow cytometry. a Autophagy flux evaluated after 48 h of AdoMet treatment. b Autophagy flux evaluated after 72 h of AdoMet treatment. The experiments were repeated at least three times and always gave similar results. The percentage of MFI was calculated as % of untreated control. Values are the means of three independent experiments.Back to article page