Fig. 5

ABF-induced apoptosis requires the JNK-mediated multisite phosphorylation of YAP. a The JNK inhibitor SP600125 antagonized the ABF-induced multisite phosphorylation of YAP. b, c Quantitative data of the indicated proteins b and the YAP shift c were analyzed. Data represent the mean ± SEM, n = 3. ^***P < 0.001 versus the control group; ^###P < 0.001 versus the ABF group. d SP600125 suppressed the interaction of YAP and p73 induced by ABF. The YAP/p73 complex was pulled down by coimmunoprecipitation with an anti-p73 antibody. e Quantitative results of the YAP/p73 complex in ABF-treated cells. Data represent the mean ± SEM, n = 3, ^**P < 0.01 versus the control; ^##P < 0.01 versus the ABF group alone. f SP600125 reversed the ABF-induced upregulation of Bax and p53AIP1. The mRNA expression levels were examined by quantitative real-time PCR. Each column represents the mean ± SEM (n = 3). g, h SP600125 antagonized the ABF-induced apoptosis in MCF-7 cells. Cells were pretreated with SP600125 and then incubated with ABF. Apoptosis (g) and cell viability (h) were analyzed. Each column represents the mean ± SEM (n = 3). ^**P < 0.01 and ^***P < 0.001 versus the control; ^#P < 0.05 and ^###P < 0.001 versus the ABF group