Fig. 3
Stable proliferation and division cycle of HXEX-ALL1 cells at different PDLs. a Growth curves of HXEX-ALL1 cells at 30, 90 and 180 PDLs. Cells were cultured in a 6-well culture plate at 6 × 105/ml in RPMI-1640 medium with 10% FBS and grown for 8 days. Viable cells were counted using trypan blue staining every day. Cell viability was evaluated by MTT assays. Experiments were performed in triplicate. b Cell cycle distribution of HXEX-ALL1 cells at 30, 90 and 180 PDLs. Cells were cultured in a 6-well culture plate at 3 × 105/ml, and cell cycle progression was analyzed by PI staining after 48 h. Experiments were performed in triplicate. The data was analyzed with the MultiCycle Software for Windows (Phoenix Flow Systems, San Diego, CA). The percentage of cells in G0/G1, G2/M and S phase was not significantly different (P > 0.05) among different PDLs