Fig. 6

Involvement of p-EGFR in Ibr-7 sensitive radiation induced apoptosis in pancreatic cancer cells. a Cells were pretreated with Ibr-7 for 24 h and then exposed to radiation for another 24 h. Western blot analysis was performed to detect the expression of p-EGFR, EGFR, β-actin was used as a loading control. b Both PANC-1 and Capan2 cells were transfected with phosphomimic mutant of EGFR plasmid and empty vector. Cells were collected after 24 h transfection and the expression of p-EGFR were detected by western blot. c The ratio of p-EGFR/EGFR was quantified by densitometry based on immunoblot images. d The ratio of p-EGFR/β-actin was quantified by densitometry based on immunoblot images. e, f The ratio of apoptosis in PANC-1 and Capan2 cells that had been transfected with phosphomimic mutant of EGFR plasmid or empty vector, then pretreated with Ibr-7 and exposed to radiation. The percentages of cell apoptosis were quantified. Results shown are the mean ± SD of 3 independent experiments. Significance was determined by Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001)