Fig. 2
From: Nanobody-armed T cells endow CAR-T cells with cytotoxicity against lymphoma cells
Nb CAR construction. A Schematic diagram of Nb CARs, including the CD8 signal peptide (SP), Nb, IgG4 hinge, CD8TM, 4-1BB, and CD3ζdomain. B, C Nb CARs expression on 293T cells. The 293T cells were transduced with control or different Nb CARs, GFP expression was observed after 72 h, cell lysates were collected and blotted with the primary antibody mouse anti-human CD3, and specific bands were observed