Fig. 3
From: Nanobody-armed T cells endow CAR-T cells with cytotoxicity against lymphoma cells
Nb CAR-T cell generation and expansion. A Schematic diagram of Nb CAR-T cells generation. Primary T cells were activated, transduced and then expanded with IL-2 up to day 10. B The bright field and fluorescence field in different groups were observed by fluorescence microscopy. C, D Nb CAR-T expression and phenotype. Modified T cells were collected on day 7, T cells were stained with 7-AAD, PE-conjugated mouse anti-human CD3, APC-Cy7TM-conjugated mouse anti-human CD8, and BV510-conjugated mouse anti-human CD4 (BD), and CAR-T cells positive expression was defined as FITC + PE+. The dead cells were excluded by 7AAD. E The expansion of Nb CAR-T cells. The cell numbers were recorded every 3 days after transduction on day 3