Fig. 2

YY1 knockdown inhibits PDAC cells proliferation. A Relative YY1 mRNA levels in MIA-PaCa2 control and YY1 KD cells (n = 3 per group) (Western blot analysis of YY1 expression in MIA-PaCa2 and YY1 KD cells; statistical analysis was based on YY1 levels corresponding to the MIA-PaCa2 control and YY1 KD cells (n = 3 per group). C Relative YY1 mRNA transcription levels in PANC1 control and YY1 KD cells (n = 3 per group). D YY1 levels in MIA-PaCa2 control and YY1 KD cells detected via western blotting; statistical analysis was based on YY1 levels corresponding to the MIA-PaCa2 control and YY1 KD cells (n = 3 per group). E Proliferation rate of the MIA-PaCa2 control and YY1 KD cells; data were normalized to cell number at t = 0. F Proliferation rate of PANC1 control and YY1 KD cells determined as in E. G (left) Colony formation in crystal violet-stained MIA-PaCa2 control and YY1 KD cells after 10 days of cell culturing, (right) Colony number counted using the ImageJ software. H Colony formation in PANC1 control and YY1 KD cells determined as in G. I Apoptosis of MIA-PaCa2 control and YY1 KD cells measured via flow cytometry (n = 3 per group). The bar graph located to the lower right depicts the statistical analysis of cell apoptosis. J Cell apoptosis of PANC1 control and YY1 KD cells determined as in I