Fig. 4

YY1 knockdown downregulates mitochondrial genome-encoded OXPHOS gene expression. A Differentially expressed genes in MIA-PaCa2 control and YY1 KD cells. The threshold was set as: P-adj < 0.05, |log2(Ctrl/YY1 KD) |> 0. B KEGG pathway enrichment analysis of metabolic genes in YY1 KD downregulated genes. The Gene Set Enrichment Analysis (GSEA) was performed based on the KEGG database (https://www.genome.jp/kegg/). The threshold was set as: log₂(Ctrl/YY1 KD) > 0. Heatmap representation of tricarboxylic acid cycle (TCA) gene expression (C), nuclear encoded OXPHOS genes expression (D), and mitochondrial genome encoded OXPHOS gene expression E in MIA-PaCa2 control and YY1 KD cells as determined via RNA-seq. The data are from two independent experiments and the values are normalized via the Z-Score and color-coded as indicated (n = 2 per group). Relative mitochondrial-encoded OXPHOS protein expression in MIA-PaCa2 control and YY1 KD cells (F) and PANC1 control and YY1 KD cells (G), as determined by RT-qPCR. β-actin was the internal control (n = 3 per group)