Fig. 5

YY1 knockdown impairs mitochondrial function in pancreatic cancer cells. A Respiratory chain single complex expression levels were determined in MIA-PaCa2 control and YY1 KD cells via BNG (CI: anti-GRIM19, CII: anti-SDHA, CIII: anti-core2, CIV: anti-COX I, CV: anti-ATP synthase subunit alpha (ATP5F1A), anti-TOM70 as loading control, n = 3 per group). B Quantification of single complex expression, based on density (n = 3 per group). C Oxygen consumption rate of the MIA-PaCa2 control and YY1 KD cells. Relative ATP levels in MIA-PaCa2 control and YY1 KD cells (n = 3 per group). Whole cell ATP level (D), cells were incubated with 5 mM 2-DG and 5 mM pyruvate for the measurement of mitochondria-generated ATP (E) and cells incubated with 5 mM glucose and 1.25 µg/mL oligomycin for the measurement of glycolysis-generated ATP (F) (n = 3 per group)