Fig. 6

Aspartate restores cell proliferation in YY1 KD cells due to impaired mitochondrial respiration. A-D Gas Chromatography/Mass Spectrometry (GC/MS) quantification of different metabolic pathway metabolites in MIA-PaCa2 control and YY1 KD cells. A Glycolysis, B pentose phosphate pathway, C one carbon, and D TCA (n = 3 per group). E Proliferation rates of MIA-PaCa2 control and YY1 KD cells treated with mitochondrial OXPHOS inhibiter, rotenone (25 nM), with or without aspartate (20 mM). Cell number was normalized with t = 0 (n = 3 per group). F Proliferation rates of MIA-PaCa2 control and YY1 KD cells were calculated with or without aspartate (20 mM). Cell number was normalized with t = 0 (n = 3 per group). G Proliferation rates of PANC1 control and YY1 KD cells determined as in F (n = 3 per group). H Proliferation rates of MIA-PaCa2 PC KD cells determined in the presence or absence of aspartate (20 mM). Cell number was normalized with t = 0 (n = 3 per group)