Fig. 4

Western-blot showed the protein expression of SPARC was reduced in LLC and B16F10 tissues treated with H₂O₂ as compared to controls, while DPI treatment resulted in an up-regulation of SPARC (A); Cell inhibition rate of HUVECs at different treatment time points (30 min, 6 and 24 h) across a range of H₂O₂ intensities (B); ELISA demonstrated that SPARC of HUVECs decreased with the duration of H₂O₂ exposure (C); Western-blot showed a significant upregulation of intracellular SPARC in the presence of H₂O₂.

SPARC: secreted acidic and cysteine-rich protein; LLC: Lewis lung carcinoma cell line; B16F10: murine melanoma cell line; HUVEC: human umbilical vein endothelial cells; ELISA: enzyme-linked immunosorbent assay