Down-regulation of cellular FLICE-inhibitory protein (Long Form) contributes to apoptosis induced by Hsp90 inhibition in human lung cancer cells
- Qilin Wang†1, 2,
- Wendong Sun†3,
- Xuexi Hao3,
- Tianliang Li1,
- Ling Su1Email author and
- Xiangguo Liu1, 4Email author
© Wang et al.; licensee BioMed Central Ltd. 2012
Received: 26 November 2012
Accepted: 20 December 2012
Published: 21 December 2012
Cellular FLICE-Inhibitory Protein (long form, c-FLIPL) is a critical negative regulator of death receptor-mediated apoptosis. Overexpression of c-FLIPL has been reported in many cancer cell lines and is associated with chemoresistance. In contrast, down-regulation of c-FLIP may drive cancer cells into cellular apoptosis. This study aims to demonstrate that inhibition of the heat shock protein 90 (Hsp90) either by inhibitors geldanamycin/17-N-Allylamino-17-demethoxygeldanamycin (GA/17-AAG) or siRNA technique in human lung cancer cells induces c-FLIPL degradation and cellular apoptosis through C-terminus of Hsp70-interacting protein (CHIP)-mediated mechanisms.
Calu-1 and H157 cell lines (including H157-c-FLIPL overexpressing c-FLIPL and control cell H157-lacZ) were treated with 17-AAG and the cell lysates were prepared to detect the given proteins by Western Blot and the cell survival was assayed by SRB assay. CHIP and Hsp90 α/β proteins were knocked down by siRNA technique. CHIP and c-FLIPL plasmids were transfected into cells and immunoprecipitation experiments were performed to testify the interactions between c-FLIPL, CHIP and Hsp90.
c-FLIPL down-regulation induced by 17-AAG can be reversed with the proteasome inhibitor MG132, which suggested that c-FLIPL degradation is mediated by a ubiquitin-proteasome system. Inhibition of Hsp90α/β reduced c-FLIPL level, whereas knocking down CHIP expression with siRNA technique inhibited c-FLIPL degradation. Furthermore, c-FLIPL and CHIP were co-precipitated in the IP complexes. In addition, overexpression of c-FLIPL can rescue cancer cells from apoptosis. When 17-AAG was combined with an anti-cancer agent celecoxib(CCB), c-FLIPL level declined further and there was a higher degree of caspase activation.
We have elucidated c-FLIPL degradation contributes to apoptosis induced by Hsp90 inhibition, suggesting c-FLIP and Hsp90 may be the promising combined targets in human lung cancer treatment.
Keywordsc-FLIPL Apoptosis CHIP Hsp90
Caspase-8 activation plays an important role in the death receptor-mediated extrinsic apoptotic pathway in human cancer cells . When binding to ligands, the death receptor is activated and forms the death induced signal complex (DISC) together with FADD and procaspase-8. Procaspase-8 is activated and initiates the caspase cascade which mediates cellular apoptosis. c-FLIPL is a major protein that can prevent caspase-8/10 activation in the DISC and inhibiting apoptosis mediated by death receptors. It has been found that c-FLIPL is up-regulated in several carcinomas [2, 3] and overexpression of c-FLIPL can be responsible for chemoresistance and malignant transformation [4–6]. To date, more than 10 splicing variants of c-FLIP genes have been identified at the mRNA level. However, only c-FLIPL and c-FLIPS have been extensively studied at the protein level. Although these two splicing variants have distinct structural and functional properties, they have been found to be recruited to DISC and to inhibit caspase-8 activation. Recent studies have shown that c-FLIPL degradation is dependent on JNK-mediated E3-ligase Itch and AKT phosphorylation, while degradation of c-FLIPS seems to have different pattern [7, 8].
Hsp90 is a pleiotropic molecular chaperone and functions as a key protein in the conformational maturation and stability of client proteins, many of which are kinases, cell cycle regulators and steroid receptors, etc. . These client proteins play important roles in signaling transduction, cell proliferation and cancer chemoresistance. The Hsp90-based molecular chaperone complex interacts with its client proteins in an iterative way. Hsp90 cycles are driven by ATP- or ADP-bound conformations that are mediated via multiple rounds of ATP binding and hydrolysis . Hsp90 inhibitors such as geldanamycin (GA) or its synthetic analogue 17-AAG, directly binds to the ATP-binding pocket in the N-terminal domain of Hsp90 and, hence, blocks the binding of nucleotides to Hsp90. Once Hsp90 activity is inhibited, the Hsp90-dependent protein dissociates from the multi-chaperone complexes and is targeted for degradation by the ubiquitin-proteasome system [10, 11]. 17-AAG-induced apoptosis has been previously reported to be associated with c-FLIPS down-regulation . However, the relationship between Hsp90 and c-FLIPL has not been well elucidated.
C-terminus of Hsp70-interacting protein (CHIP) has the E3 ligase activity, and it binds to Hsp/Hsc70 and Hsp90 complex by means of its TPR (an amino-terminal tetratricopeptide) domain . It has been reported that CHIP degrades Hsp90 client proteins, such as the glucocorticoid receptor, the cystic fibrosis transmembrane-conductance regulator and ErbB2 [13–15]. In this study, we show that CHIP is involved in c-FLIPL degradation induced by Hsp90 inhibition. Since both Hsp90 and c-FLIPL play a critical role in oncogenesis and chemoresistance, inhibition of Hsp90 by 17-AAG in combination with therapeutic agents targeting c-FLIP may be an efficacious strategy in patients with tumors that express high levels of c-FLIPL and other Hsp90 client proteins.
Hsp90 inhibitor 17-AAG and GA induces c-FLIPL down-regulation via ubiquitin-proteasome pathway in NSCLC cells
Inhibition of Hsp90 expression induces c-FLIPL down-regulation
CHIP modulates the c-FLIPL degradation induced by 17-AAG
CHIP interacts with c-FLIPL in vivo and promotes the ubiquitination of c-FLIPL
To further test if there is physically interaction between CHIP and c-FLIPL, immunoprecipitation experiments were performed. Calu-1 cells were transfected with plasmids carrying Myc-tagged CHIP or Flag-tagged c-FLIPL and then treated with 1.0 μM 17-AAG for 4 h, the lysate supernatant was incubated with Myc antibody for 1 h and then with protein-G and protein-A (1:1 mix) beads overnight (left panel), or anti-FLAG M2 Affinity Gel beads overnight directly(right panel). Western Blot analysis showed that CHIP and c-FLIPL proteins were both detected in the immunoprecipitation complexes, suggesting that both exogenous and endogenous CHIP interacts with c-FLIPL physically in vivo. Hsp90 was also pulled down together with c-FLIPL and CHIP, suggesting that CHIP interacts with c-FLIPL in an Hsp90 chaperone complex (Figure 4D). Considering CHIP can be an E3 ligase, we wonder if it can regulate the ubiquitination level of c-FLIPL. H157-c-FLIPL cells were transfected with plasmids carrying Myc-tagged CHIP or Myc-tagged CHIP (H260Q Mutant) and then treated with 1.0 μM 17-AAG for 8 h. After immunoprecipitaion with Flag antibody, Flag-tagged c-FLIPL was pulled down. Western Blot analysis showed that CHIP overexpression promoted the c-FLIPL ubiquitination, while the mutant CHIP overexpression suppressed the c-FLIPL ubiquitination after 17-AAG treatment (Figure 4E). Taken together, our data suggest that CHIP interacts with c-FLIPL in vivo and promotes the ubiquitination of c-FLIPL.
Ectopic overexpression of c-FLIPL protects cancer cells from apoptosis
17-AAG enhances celecoxib-induced c-FLIPL down-regulation and apoptosis
Hsp90 plays a key role in conformational maturation and stability of client proteins in transducing proliferative and anti-apoptotic signals . In the present study we found that Hsp90 inhibitor 17-AAG and GA or Hsp90 knockdown induced c-FLIPL down-regulation which subsequently resulted in cancer cell apoptosis. Although DR5 is an important death receptor in the extrinsic apoptotic pathway, in the above experiments, we failed to detect DR5 up-regulation (data no shown). This indicates that c-FLIPL may primarily contribute to Hsp90 inhibition-induced apoptosis in lung cancer cells.
Currently, more than one hundred client proteins of Hsp90 have been identified, and they are degraded when Hsp90 multi-chaperone complex activity is disrupted by the inhibitors. When we inhibited Hsp90 expression using siRNA technique and examined the c-FLIPL level, we found c-FLIPL was reduced in the cells whose Hsp90 was knocked down, suggesting that c-FLIPL is a client protein of Hsp90. It is reported when Hsp90 is inhibited, it recruits E3 ligase (e.g. CHIP) to degrade the client proteins [13–15]. When we knocked down CHIP, c-FLIPL degradation was inhibited after treatment with 17-AAG, which indicated that CHIP modulated c-FLIPL degradation in the NSCLC cell lines. Next, we performed immunoprecipitation experiments in Calu-1 cell lines. We found that CHIP was co-precipitated with c-FLIPL in the precipitated complex. We also found that Hsp90 was precipitated at the same time, hinting that c-FLIPL is the client protein of Hsp90 and CHIP modulates c-FLIPL degradation through the Hsp90 chaperone complex. Itch is reported as an E3 ligase of c-FLIPL in the mouse cells upon exposure to TNFα . However, it has also been reported that some agents induced c-FLIPL degradation independently of Itch [17–19]. Additionally, we found that CHIP promotes the ubiquitination of c-FLIP, indicating CHIP regulates c-FLIPL degradation through uibiquitination when the cells were treated with 17-AAG.
It has been investigated that the clinically used anti-inflammatory drug celecoxib induces c-FLIPL down-regulation and facilitates cancer cell apoptosis alone or in combination with other agents . We found that 17-AAG and celecoxib had synergistic effects in promoting c-FLIPL down-regulation and cellular apoptosis. In addition, overexpression of c-FLIPL could rescue cancer cells from apoptosis. These data suggest that c-FLIPL down-regulation plays important role in Hsp90 inhibition-induced apoptosis in NSCLC cells.
Our data reveals that c-FLIPL is down-regulated and apoptosis is induced effectively by Hsp90 inhibition, either by 17AAG and GA or siRNA technique, and c-FLIPL is degraded via proteasome-mediated pathway. CHIP modulates c-FLIP degradation induced by Hsp90 inhibition in human NSCLC cell lines. Targeting both c-FLIPL and Hsp90 may represent a promising therapeutic strategy for the patients whose tumors overexpress c-FLIPL and Hsp90 client proteins.
Materials and methods
Reagents and antibodies
17-AAG and GA were purchased from LC Laboratories (Pompano Beach). Celecoxib, MG132 and Anti-FLAG M2 Affinity Gel were purchased from Sigma (St. Louis, MO). Protein A and Protein G Agarose were purchased from Roche (Mannheim, Germany).
Mouse anti-caspase-3 and anti-caspase-8 monoclonal antibodies, rabbit anti-caspase-9 and anti-poly (ADP-ribose) polymerase (PARP) antibodies were purchased from Cell Signaling Technology (Danvers, MA). Mouse anti-FLIPL monoclonal antibody was purchased from Alexis Biochemicals (San Diego, CA). Mouse anti-Hsp90 antibodies were purchased from Abcam (Cambridge, UK). Mouse anti-Myc and anti-β-actin antibodies, rabbit anti-Flag and anti-CHIP antibodies were purchased from Sigma (St. Louis, MO).
Cell lines, cell culture and transfection
The human non-small cell lung cancer cell lines used in this study were obtained from the American Type Culture Collection (Manassas, VA) and were grown in RPMI 1640 supplemented with 5% fetal bovine serum at 37°C in a humidified atmosphere of 5% CO2 and 95% air. Cells were transfected with different plasmids by using the FuGene reagent from Roche (Indianapolis, IN), according to the manufacturer’s instructions.
Cell survival assay
Cells were seeded in 96-well plate with an appropriate amount in 100 μL culture medium per well. On the second day, compounds or drugs were mixed in and the total volume of per well was 200 μL. Cells were treated for defined time and then cell viability was measured by sulforhodamine B (SRB) assay as described previously .
Western blot analysis
The whole-cell protein lysates preparation and Western blot analysis were described in reference .
Plasmids and establishment of stable cell lines that overexpress c-FLIPL
Plasmids of Myc-CHIP and Myc-CHIP (H260Q) were kindly provided by Dr. Hamid Band (University of Nebraska Medical Center, USA). Lenti-Flag-c-FLIPL was constructed before . The cell clones that can overexpress c-FLIPL and LacZ were constructed as previously described .
Knockdown of Hsp90, CHIP expression with siRNA
siRNAs were synthesized by GenePharma (Shanghai, China). Control siRNA and Hsp90α/β siRNA target sequences were described before . The target sequence of CHIP siRNA was 5′-AGGCCAAGCACGACAAGTA-3′. Transfection of siRNA was conducted as previously described .
To precipitate Flag-tagged FLIPL or Myc-tagged CHIP proteins, cells were lysed with lysis buffer and Anti-FLAG M2 Affinity Gel beads or Myc antibody with Protein A/G Agarose beads (1:1 mix) were used to pull down Flag-tagged FLIPL or Myc-tagged CHIP proteins according to the manufacture’s instructions.
We thank Dr. Hamid Band (University of Nebraska Medical Center, USA) for kindly providing the plasmids of Myc-CHIP and Myc-CHIP (H260Q). We thank Dr. Sunil Singhal for English editing.
This work was supported by grants from the National Natural Science Foundation of China (31071215 and 30971307), National Science Major Project from Ministry of Science and Technology of China (2013CB910900 project 3) and Shandong Provincial Natural Science Foundation (2010GSF10218 and JQ201007).
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