- Primary research
- Open Access
ERK-mediated autophagy promotes inactivated Sendai virus (HVJ-E)-induced apoptosis in HeLa cells in an Atg3-dependent manner
- Tao Wang†1, 2,
- Ning Yu†1, 2,
- Miao Qian1, 2, 4,
- Jie Feng3,
- Shuyang Cao1, 2,
- Jun Yin1, 2 and
- Quan Zhang1, 2Email author
© The Author(s) 2018
- Received: 19 July 2018
- Accepted: 27 November 2018
- Published: 4 December 2018
Apoptosis and autophagy are known to play important roles in cancer development. It has been reported that HVJ-E induces apoptosis in cancer cells, thereby inhibiting the development of tumors. To define the mechanism by which HVJ-E induces cell death, we examined whether HVJ-E activates autophagic and apoptotic signaling pathways in HeLa cells.
Cells were treated with chloroquine (CQ) and rapamycin to determine whether autophagy is involved in HVJ-E-induced apoptosis. Treatment with the ERK inhibitor, U0126, was used to determine whether autophagy and apoptosis are mediated by the ERK pathway. Activators of the PI3K/Akt/mTOR/p70S6K pathway, 740 Y-P and SC79, were used to characterize its role in HVJ-E-induced autophagy. siRNA against Atg3 was used to knock down the protein and determine whether it plays a role in HVJ-E-induced apoptosis in HeLa cells.
We found that HVJ-E infection inhibited cell viability and induced apoptosis through the mitochondrial pathway, as evidenced by the expression of caspase proteins. This process was promoted by rapamycin treatment and inhibited by CQ treatment. HVJ-E-induced autophagy was further blocked by 740 Y-P, SC79, and U0126, indicating that both the ERK- and the PI3K/Akt/mTOR/p70S6K-pathways were involved. Finally, autophagy-mediated apoptosis induced by HVJ-E was inhibited by siRNA-mediated Atg3 knockdown.
In HeLa cells, HVJ-E infection triggered autophagy through the PI3K/Akt/mTOR/p70S6K pathway in an ERK1/2-dependent manner, and the induction of autophagy promoted apoptosis in an Atg3-dependent manner.
- HeLa cell
Cervical cancer is the third most commonly diagnosed cancer in women globally, and malignant cervical neoplasias are the second most common cause of death among women . Currently, there exist several methods to treat cervical cancer, including surgical therapy , gene therapy , immunity therapy , radiotherapy , and chemotherapy . However, tumors can be resistant to certain types of available therapies, including chemotherapy, thereby increasing the difficulty of acquiring sufficient treatment . New therapeutic options are urgently required in order to meet these treatment needs. Oncolytic virus infection has shown great potential as a new cancer treatment method , and several oncolytic viruses have been identified and developed as safe and effective therapeutic tools . Presumably, tumors are infected with oncolytic viruses which then lyse and kill the cancerous cell. A previous study has reported that cervical carcinoma cells are sensitive to the vesicular stomatitis virus, and that cells infected with the human papilloma virus are receptive to oncolytic virus therapy . In recent years, inactivated Sendai virus particles (hemagglutinating virus of Japan envelope, HVJ-E) have been shown to contribute to several anti-cancer effects, such as the activation of anti-tumor immunity via anti-tumorigenic neutrophils in the tumor microenvironment , the suppression of murine melanoma growth by host immune response, and the down-regulation of beta-catenin expression .
Apoptosis is the principal mechanism behind programmed cell death, and apoptosis functions through several complex biochemical and genetic pathways. Apoptosis plays a critical role during the development and aging in normal tissues, which contributes to the healthy balance between cell survival and cell death [13, 14]. Insufficient apoptosis typically results in cancer or autoimmunity, while accelerated cell death is a hallmark of many diseases . Recently, HVJ-E was found to promote apoptosis in various cancer cells, including murine melanoma cells and human prostate cancer PC3 cells [16, 17]. HVJ-E was also found to induce autophagy in human lung cancer cells .
Autophagy is reported as a cellular survival strategy that eliminates intracellular proteins and organelles to sustain metabolic balance in cells [19, 20]. However, an increasing pool of evidence indicates that autophagy is a regulated programmed death process, which is closely associated with the development of tumors. It has been demonstrated that autophagy is involved in tumor suppression during the early stages of cancer development [21, 22]. While some models have shown that cancer initiation is suppressed by autophagy, it is also true that autophagy provides nutrients that support the growth of advanced malignant tumors [23, 24]. The exact role of autophagy in tumor cells may be dependent on the type of tumor, the stage of tumorigenesis, or the nature and extent of the insult to the cell . Thus, it is important to clarify the relationship between autophagy and apoptosis as a prelude to tumor suppression.
It has been reported that the PI3K/Akt/mTOR/p70S6K signaling pathway is involved in regulation of the cell cycle, cellular transformation, tumorigenesis, and autophagy during chemotherapy [26, 27]. Moreover, the mitogen-activated protein kinase (MAPK) signaling pathway has been shown to induce autophagy in various cancer cells . The extracellular signal-regulated kinase (ERK) signaling pathway has been identified as a player in the initiation of both autophagy and apoptosis induced by deprivation of amino acids or treatment with aurintricarboxylic acid, β-group soyasaponins, or curcumin [29–31].
Although apoptosis and autophagy can be determined alternatively [26, 27], the question remains as to whether autophagy is induced by a separate death effector mechanism independent of apoptosis, or whether it is a trigger for or dependent upon apoptosis [32, 33]. Autophagy has been shown in some cases to both promote caspase-independent cell death and modulate caspase-mediated apoptosis [34–38]. Moreover, autophagy and apoptosis share common regulators, such as Ca2+, reactive oxygen species (ROS), and the presence of endoplasmic reticulum (ER) stress [32, 39]. Studies have shown that ERK activity can promote either intrinsic or extrinsic apoptotic pathways by triggering mitochondrial cytochrome c release or caspase-8 activation . However, it remains unclear whether autophagy is involved in ERK-mediated apoptosis.
Atg12 can modify multiple protein targets in mammalian cells, which has been identified to conjugate to Atg5 in autophagy process. Atg3, an E2-like enzyme that conjugates Atg8 to phosphatidylethanolamine (PE), has been identified as a second target of Atg12 conjugation. The Atg12–Atg3 complex has been found to regulate the early steps of autophagy, and the disruption of the Atg12–Atg3 conjugation has profound effects on mitochondrial function . In this study, we aimed to explore the molecular mechanisms behind autophagy-related apoptosis in cancer cells, which could provide novel insights into developing novel cancer therapies.
Cells, plasmids, and virus
HeLa cells were purchased from the Institute of Biochemistry and Cell Biology in Shanghai, China. GFP-microtubule-associated protein 1 light chain 3 (GFP-LC3) plasmids were kindly provided by Dr. Songshu Meng (Dalian Medical University, Dalian, China). Sendai virus (Z strain) samples were harvested from the chorioallantoic fluid of 10–14 day old chick eggs, purified by centrifugation, and inactivated by UV irradiation (99 mJ/cm2), as described previously .
Antibodies and reagents
Antibodies used in this study were purchased from Cell Signaling Technology and included: caspase-3, caspase-9, phospho-mTOR, phospho-Akt, phospho-p70S6K, phospho-JNK, phospho-p38, phospho-ERK1/2, total mTOR, total Akt, total p70S6K, total JNK, total p38, total ERK1/2, Beclin 1, p62, p53, Bcl-2, Bax, Atg3, β-actin, and PARP. The pan-caspase inhibitor Z-VAD-FMK was purchased from Promega and the specific inhibitors of MEK (U0126), The polyclonal rabbit anti-microtubule-associated protein 1A/1B-light chain 3 (LC3) antibody, rapamycin (rap), chloroquine (CQ), and horseradish peroxidase (HRP)-conjugated goat anti-rabbit immunoglobulin were obtained from Sigma-Aldrich. The FITC-Annexin V Apoptosis Detection Kit I used in the study was purchased from BD Bioscience.
Cell culture and morphological changes
HeLa cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum in a humidified cell incubator with an atmosphere of 5% CO2 at 37 °C. HeLa cells were treated with the indicated multiplicity of infection (MOI) of HVJ-E for 24 h, and then morphological changes in the cells were photographed using an inverted microscope (DMI 3000B, Leica) at 200× magnification.
Cell viability assay
Cells were seeded into 96-well plates at a density of 1 × 104 cells per well and incubated for 24 h. C-terminal octapeptide of cholecystokinin (CCK-8) assays (Beyotime, Shanghai, China) were used to assess cell viability after infection with various MOIs. The optical density (OD) at 450 nm was read using a Bio-Tek ELISA microplate reader. The viability rate was calculated as the ratio of the OD in experimental wells to the OD in normal wells.
Analysis of apoptosis using flow cytometry
Samples containing 5 × 105 cells were treated with HVJ-E for 24 h at the following MOIs: 0, 100, 200, 400, and 800. Cells were harvested and then stained with Annexin V-FITC and PI according to the manufacturer’s instructions. For the apoptosis assays, HeLa cells were incubated with either Z-VAD-FMK inhibitor, rapamycin, or CQ for 40 min, prior to HVJ-E treatment at 800 MOI. The percentage of apoptotic cells was then determined by flow cytometry.
GFP-LC3 transfection and fluorescence microscopy
The GFP-LC3 plasmids were transfected into HeLa cells using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s guidelines. The formation of GFP-LC3 puncta was observed under a fluorescence microscope (DMI 3000B, Leica) after cells were treated with the rapamycin and HVJ-E as indicated. Cells with five or more puncta were considered to have accumulated autophagosomes, because up to four puncta were observed in a small number of untreated cells. A total of 100 transfected cells were analyzed in each well, and three independent experiments were performed.
Western blot analysis
HeLa cells were treated with HVJ-E for 24 h. Proteins were extracted from infected and non-infected cells using a cell lysis buffer. Proteins were then separated by 8–15% SDS-PAGE and transferred to a PVDF membrane. The membranes were blocked in 5% non-fat milk for 1 h before incubation with primary antibody (1:1000) overnight at 4 °C. The bound antibody complexes were detected using a chemiluminescence reagent after membranes were incubated with HRP-conjugated IgG secondary antibodies (1:5000).
All studies were performed as three independent experiments. The data are expressed as mean ± SD. Significant variance between groups was determined using one-way ANOVA. Differences of P < 0.05 were considered statistically significant.
HVJ-E inhibited cell viability and induced apoptotic cell death in HeLa cells
HVJ-E induced mitochondrial apoptosis in HeLa cells in a caspase-dependent manner
HVJ-E induced autophagic flux in HeLa cells
HVJ-E induced autophagy in HeLa cells in a PI3K/Akt/mTOR-dependent manner
ERK1/2 regulates HVJ-E-induced autophagy by inhibiting the PI3K/AKT/mTOR pathway
HVJ-E-induced apoptosis was promoted by the ERK-autophagy pathway in HeLa cells
To investigate whether the ERK-autophagy signaling pathway is involved in apoptosis induced by HVJ-E, cells were treated with U0126 to inhibit ERK1/2. As shown in Fig. 6d, e, pre-treatment with U0126 resulted in a reduction of apoptosis induced by HVJ-E and a decrease in cleaved-caspase-3 expression levels (Fig. 6f, g). Taken together, these results show that HVJ-E-induced apoptosis was promoted by the activation of the ERK-autophagy pathway in HeLa cells.
HVJ-E-induced apoptosis was inhibited by Atg3 knock down in HeLa cells
HVJ-E infection has been shown to activate host immune response  and promote apoptosis in cancer cells . It is therefore feasible that HVJ-E could be a medium for cancer treatments. Studies have shown that apoptosis induced by HVJ-E infection is associated with the activation of caspase-8 in PC3 human prostate cancer cells  and the activation of caspase-9 in murine B16F10 melanoma cells . However, it was also found that cell death in human neuroblastomas is due to necrosis  rather than apoptosis . Based on this evidence, it seemed that whether HVJ-E infection induces apoptosis or necrosis is dependent on the cell type. In this study, we found that HVJ-E induces apoptosis in HeLa cells via the caspase-dependent mitochondrial pathway. We also showed for the first time that autophagic activation promotes apoptosis induced by HVJ-E. Our results demonstrate that, in HeLa cells, HVJ-E induces autophagy through the activation of the ERK pathway, which subsequently leads to the induction of apoptosis via Atg3.
Regulation of autophagy is thought to be a powerful therapeutic strategy in the treatment of various cancers. In fact, many anti-cancer drugs and naturally occurring compounds are reported to have anti-tumor effects brought on by promoting autophagy-dependent apoptotic cell death or senescence in various types of cells. Studies have shown that autophagic flux was blocked in senescent mesenchymal stromal cells (MSCs), indicating that autophagy is closely linked to senescence . Moreover, microRNA (miRNA)-494 induced senescence in human lung cancer cells while suppressing the development of tumor . Which indicates that cell senescence plays an important role in anti-cancer therapy Like cellular senescence, apoptosis is an extreme response to cellular stress, and it represents an important tumor-suppressive mechanism. Our results show that HVJ-E induces apoptosis rather than senescence in HeLa cells, indicating that the fate of cells depends entirely on cell type and their ability to cope with stress. However, further study is needed to identify whether tumor development can be suppressed by HVJ-E infection in vivo.
Despite the existence of of several studies characterizing HVJ-E, there is yet little known about the mechanisms by which HVJ-E induces autophagy in HeLa cells. The current understanding of autophagy is that its induction can result in either cytoprotection or cell death. We have shown that both apoptosis and autophagy are induced by HVJ-E in HeLa cells and that the two are linked. We found that apoptosis was enhanced by rapamycin treatment and inhibited by CQ treatment in HVJ-E-infected HeLa cells. This data indicates that autophagy may act as a death mechanism. This result is consistent with a study performed in A549 cells, wherein it was suggested that inducing autophagy enhances apoptosis, and conversely, inhibition of autophagy suppresses apoptosis triggered by HVJ-E. Interestingly, 3-methyladenine (3-MA), a known inhibitor of autophagy suppressed autophagy and effectively increase the rate of apoptosis in HeLa cells. Therefore, autophagy may play two distinct and opposite roles in apoptosis.
ERK1/2 has been identified as the regulator of apoptosis in many cell types [52, 53]. Cagnol and coworkers found that ERK activity promoted either intrinsic or extrinsic apoptotic pathways and autophagic vacuolization . In our study, we found evidence suggesting that the ERK1/2 signaling pathway induces autophagy in HVJ-E-infected HeLa cells, which subsequently leads to cell death. Many studies have reported that the PI3K/AKT/mTOR pathway mediates the induction of autophagy and apoptosis. Saiki et al. reported that inhibition of the PI3K/AKT/mTOR pathway promotes caffeine-induced apoptosis by enhancing autophagy levels . We have found that HVJ-E-induced autophagy regulated by ERK1/2 is PI3K/AKT/mTOR pathway-dependent. Furthermore, we found the expression of p53 to be activated. Several studies have shown that ERK-mediated p53 expression is required for apoptosis .
Recently, JNK activation has been reported to contribute to autophagy and apoptosis via the regulation of Beclin 1/Bcl-2 interaction . Detailed studies of the role this pathway plays in cell death could contribute to its use in novel therapeutic strategies in the future. Studies have also shown that Atg3 activation contributes to apoptosis through regulation of the mitophagy pathway [41, 56]. In this study, we found that both autophagy levels and the rate of mitochondria-mediated apoptosis induced by HVJ-E infection are decreased by knocking down Atg3. This suggests that HVJ-E-induced apoptosis is promoted by autophagy via Atg3. However, we cannot exclude the possible involvement of mitophagy in the protective mechanism. Thus, the exact level of autophagy and whether other apoptotic pathways—including those upstream of apoptosis—are activated require further investigation.
The results of this study show that HVJ-E infection induces apoptosis in HeLa cells through the mitochondrial pathway, and that this induction is triggered by PI3K/Akt/mTOR/P70SK-mediated autophagy in an ERK-dependent manner. Moreover, the induction of autophagy in HeLa cells promotes HVJ-E-mediated apoptosis, and the inhibition of autophagy protects cells from apoptosis. These findings provide a molecular basis for understanding HVJ-E-mediated cell death and support the notion that combination treatment using an autophagy enhancer is an effective strategy to augment the cytotoxic effects in HeLa cells. These results provide new insight into the mechanisms behind the anti-tumor effects of HVJ-E infection.
TW: data management, data analysis, manuscript writing. NY: data analysis, project development. MQ, JF: manuscript editing. SC, JY: data collection. QZ: manuscript writing. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
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This work was supported by the National Key Research and Development Program of China (2017YFD0502303), the National Natural Science Foundation of China (31802260), the High-end Talent Support Program of Yangzhou University, the Young and Middle-aged Academic Leaders Plan of Yangzhou University, and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
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- Hernandez-Hernandez DM, Apresa-Garcia T, Patlan-Perez RM. Epidemiological overview of uterine cervical cancer. Rev Med Inst Mex Seguro Soc. 2015;53(Suppl 2):S154–61.PubMedGoogle Scholar
- Hockel M. New concepts for surgical therapy of cervical carcinoma. Pathologe. 2005;26(4):276–82.View ArticlePubMedGoogle Scholar
- Liu B, Han SM, Tang XY, Han L, Li CZ. Cervical cancer gene therapy by gene loaded PEG-PLA nanomedicine. Asian Pac J Cancer Prev. 2014;15(12):4915–8.View ArticlePubMedGoogle Scholar
- Lazarev AF, Kenbaeva DK, Medeubaev RK, Gorbatenko AE, Tanatarov SZ. Effect of immunotherapy on the cellular immunity in patients with cervical cancer. Vestn Ross Akad Med Nauk. 2014;3–4:5–8.View ArticleGoogle Scholar
- Papadopoulou I, Stewart V, Barwick TD, Park WH, Soneji N, Rockall AG, Bharwani N. Post-radiation therapy imaging appearances in cervical carcinoma. Radiographics. 2016;36(2):538–53.View ArticlePubMedGoogle Scholar
- He D, Duan C, Chen J, Lai L, Chen J, Chen D. The safety and efficacy of the preoperative neoadjuvant chemotherapy for patients with cervical cancer: a systematic review and meta analysis. Int J Clin Exp Med. 2015;8(9):14693–700.PubMedPubMed CentralGoogle Scholar
- Zhang C, Jiang Y, Zhang J, Huang J, Wang J. 8-p-Hdroxybenzoyl tovarol induces paraptosis like cell death and protective autophagy in human cervical cancer HeLa cells. Int J Mol Sci. 2015;16(7):14979–96.View ArticlePubMedPubMed CentralGoogle Scholar
- Ning J, Wakimoto H. Oncolytic herpes simplex virus-based strategies: toward a breakthrough in glioblastoma therapy. Front Microbiol. 2014;5:303.View ArticlePubMedPubMed CentralGoogle Scholar
- Fukuhara H, Ino Y, Todo T. Oncolytic virus therapy: a new era of cancer treatment at dawn. Cancer Sci. 2016;107(10):1373–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Le Boeuf F, Niknejad N, Wang J, Auer R, Weberpals JI, Bell JC, Dimitroulakos J. Sensitivity of cervical carcinoma cells to vesicular stomatitis virus-induced oncolysis: potential role of human papilloma virus infection. Int J Cancer. 2012;131(3):E204–15.View ArticlePubMedGoogle Scholar
- Chang CY, Tai JYA, Li SM, Nishikawa T, Kaneda Y. Virus-stimulated neutrophils in the tumor microenvironment enhance T cell-mediated anti-tumor immunity. Oncotarget. 2016;7(27):42195–207.View ArticlePubMedPubMed CentralGoogle Scholar
- Gao H, Xu XS, Chen ZD, Zhang Q, Xu XM. Inactivated Sendai virus induces apoptosis in murine melanoma cells by IGF-1R down-regulation. Biomed Environ Sci. 2013;26(12):998–1002.PubMedGoogle Scholar
- Ouyang L, Shi Z, Zhao S, Wang FT, Zhou TT, Liu B, Bao JK. Programmed cell death pathways in cancer: a review of apoptosis, autophagy and programmed necrosis. Cell Prolif. 2012;45(6):487–98.View ArticlePubMedGoogle Scholar
- Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol. 2007;35(4):495–516.View ArticlePubMedPubMed CentralGoogle Scholar
- Hassan M, Watari H, AbuAlmaaty A, Ohba Y, Sakuragi N. Apoptosis and molecular targeting therapy in cancer. Biomed Res Int. 2014. https://doi.org/10.1155/2014/15084.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang Q, Xu X, Yuan Y, Gong X, Chen Z, Xu X. IPS-1 plays a dual function to directly induce apoptosis in murine melanoma cells by inactivated Sendai virus. Int J Cancer. 2014;134(1):224–34.View ArticlePubMedGoogle Scholar
- Gao H, Gong XC, Chen ZD, Xu XS, Zhang Q, Xu XM. Induction of apoptosis in hormone-resistant human prostate cancer PC3 cells by inactivated Sendai virus. Biomed Environ Sci. 2014;27(7):506–14.PubMedGoogle Scholar
- Kurooka M, Kaneda Y. Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells. Cancer Res. 2007;67(1):227–36.View ArticlePubMedGoogle Scholar
- White E. The role for autophagy in cancer. J Clin Invest. 2015;125(1):42–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Denton D, Nicolson S, Kumar S. Cell death by autophagy: facts and apparent artefacts. Cell Death Differ. 2012;19(1):87–95.View ArticlePubMedGoogle Scholar
- Ng G, Huang J. The significance of autophagy in cancer. Mol Carcinog. 2005;43(4):183–7.View ArticlePubMedGoogle Scholar
- Ogier-Denis E, Codogno P. Autophagy: a barrier or an adaptive response to cancer. Biochem Biophys Acta. 2003;1603(2):113–28.PubMedGoogle Scholar
- Cheong H. Integrating autophagy and metabolism in cancer. Arch Pharm Res. 2015;38(3):358–71.View ArticlePubMedGoogle Scholar
- Kondo Y, Kanzawa T, Sawaya R, Kondo S. The role of autophagy in cancer development and response to therapy. Nat Rev Cancer. 2005;5(9):726–34.View ArticlePubMedGoogle Scholar
- Xi G, Hu X, Wu B, Jiang H, Young CY, Pang Y, Yuan H. Autophagy inhibition promotes paclitaxel-induced apoptosis in cancer cells. Cancer Lett. 2011;307(2):141–8.View ArticlePubMedGoogle Scholar
- Harder LM, Bunkenborg J, Andersen JS. Inducing autophagy: a comparative phosphoproteomic study of the cellular response to ammonia and rapamycin. Autophagy. 2014;10(2):339–55.View ArticlePubMedGoogle Scholar
- Booth LA, Tavallai S, Hamed HA, Cruickshanks N, Dent P. The role of cell signalling in the crosstalk between autophagy and apoptosis. Cell Signal. 2014;26(3):549–55.View ArticlePubMedGoogle Scholar
- Corcelle E, Djerbi N, Mari M, Nebout M, Fiorini C, Fenichel P, Hofman P, Poujeol P, Mograbi B. Control of the autophagy maturation step by the MAPK ERK and p38: lessons from environmental carcinogens. Autophagy. 2007;3(1):57–9.View ArticlePubMedGoogle Scholar
- Ogier-Denis E, Pattingre S, El Benna J, Codogno P. Erk1/2-dependent phosphorylation of Galpha-interacting protein stimulates its GTPase accelerating activity and autophagy in human colon cancer cells. J Biol Chem. 2000;275(50):39090–5.View ArticlePubMedGoogle Scholar
- Pattingre S, Bauvy C, Codogno P. Amino acids interfere with the ERK1/2-dependent control of macroautophagy by controlling the activation of Raf-1 in human colon cancer HT-29 cells. J Biol Chem. 2003;278(19):16667–74.View ArticlePubMedGoogle Scholar
- Ellington AA, Berhow MA, Singletary KW. Inhibition of Akt signaling and enhanced ERK1/2 activity are involved in induction of macroautophagy by triterpenoid B-group soyasaponins in colon cancer cells. Carcinogenesis. 2006;27(2):298–306.View ArticlePubMedGoogle Scholar
- Eisenberg-Lerner A, Bialik S, Simon HU, Kimchi A. Life and death partners: apoptosis, autophagy and the cross-talk between them. Cell Death Differ. 2009;16(7):966–75.View ArticlePubMedGoogle Scholar
- Grishchuk Y, Ginet V, Truttmann AC, Clarke PG, Puyal J. Beclin 1-independent autophagy contributes to apoptosis in cortical neurons. Autophagy. 2011;7(10):1115–31.View ArticleGoogle Scholar
- Chen Y, McMillan-Ward E, Kong J, Israels SJ, Gibson SB. Oxidative stress induces autophagic cell death independent of apoptosis in transformed and cancer cells. Cell Death Differ. 2008;15(1):171–82.View ArticlePubMedGoogle Scholar
- Zaidi AU, McDonough JS, Klocke BJ, Latham CB, Korsmeyer SJ, Flavell RA, Schmidt RE, Roth KA. Chloroquine-induced neuronal cell death is p53 and Bcl-2 family-dependent but caspase-independent. J Neuropathol Exp Neurol. 2001;60(10):937–45.View ArticlePubMedGoogle Scholar
- Yousefi S, Perozzo R, Schmid I, Ziemiecki A, Schaffner T, Scapozza L, Brunner T, Simon HU. Calpain-mediated cleavage of Atg5 switches autophagy to apoptosis. Nat Cell Biol. 2006;8(10):1124–32.View ArticlePubMedGoogle Scholar
- Scott RC, Juhasz G, Neufeld TP. Direct induction of autophagy by Atg1 inhibits cell growth and induces apoptotic cell death. Curr Biol. 2007;17(1):1–11.View ArticlePubMedPubMed CentralGoogle Scholar
- Wang Y, Han R, Liang ZQ, Wu JC, Zhang XD, Gu ZL, Qin ZH. An autophagic mechanism is involved in apoptotic death of rat striatal neurons induced by the non-N-methyl-d-aspartate receptor agonist kainic acid. Autophagy. 2008;4(2):214–26.View ArticlePubMedGoogle Scholar
- Maiuri MC, Zalckvar E, Kimchi A, Kroemer G. Self-eating and self-killing: crosstalk between autophagy and apoptosis. Nat Rev Mol Cell Biol. 2007;8(9):741–52.View ArticlePubMedGoogle Scholar
- Cagnol S, Chambard JC. ERK and cell death: mechanisms of ERK-induced cell death–apoptosis, autophagy and senescence. FEBS J. 2010;277(1):2–21.View ArticlePubMedGoogle Scholar
- Radoshevich L, Debnath J. ATG12-ATG3 and mitochondria. Autophagy. 2011;7(1):109–11.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang Q, Wang Z, Yuan Y, Xue Z, Zhai G, Zuo W, Zhu S, Zhu G, Xu X. Immunoadjuvant effects of hemagglutinating virus of Japan envelope (HVJ-E) on the inactivated H9 subtype avian influenza virus vaccine. Vet Immunol Immunopathol. 2011;141(1–2):116–23.View ArticlePubMedGoogle Scholar
- Zuo H, Lin T, Wang D, Peng R, Wang S, Gao Y, Xu X, Li Y, Wang S, Zhao L, et al. Neural cell apoptosis induced by microwave exposure through mitochondria-dependent caspase-3 pathway. Int J Med Sci. 2014;11(5):426–35.View ArticlePubMedPubMed CentralGoogle Scholar
- Cuyas E, Corominas-Faja B, Joven J, Menendez JA. Cell cycle regulation by the nutrient-sensing mammalian target of rapamycin (mTOR) pathway. Methods Mol Biol. 2014;1170:113–44.View ArticlePubMedGoogle Scholar
- Wang J, Whiteman MW, Lian H, Wang G, Singh A, Huang D, Denmark T. A non-canonical MEK/ERK signaling pathway regulates autophagy via regulating Beclin 1. J Biol Chem. 2009;284(32):21412–24.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang Q, Zhu H, Xu X, Li L, Tan H, Cai X. Inactivated Sendai virus induces apoptosis and autophagy via the PI3K/Akt/mTOR/p70S6K pathway in human non-small cell lung cancer cells. Biochem Biophys Res Commun. 2015;465(1):64–70.View ArticlePubMedGoogle Scholar
- Kawaguchi Y, Miyamoto Y, Inoue T, Kaneda Y. Efficient eradication of hormone-resistant human prostate cancers by inactivated Sendai virus particle. Int J Cancer. 2009;124(10):2478–87.View ArticlePubMedGoogle Scholar
- Nikoletopoulou V, Markaki M, Palikaras K, Tavernarakis N. Crosstalk between apoptosis, necrosis and autophagy. Biochim Biophys Acta. 2013;1833(12):3448–59.View ArticlePubMedGoogle Scholar
- Nomura M, Ueno A, Saga K, Fukuzawa M, Kaneda Y. Accumulation of cytosolic calcium induces necroptotic cell death in human neuroblastoma. Cancer Res. 2014;74(4):1056–66.View ArticlePubMedGoogle Scholar
- Capasso S, Alessio N, Squillaro T, Di Bernardo G, Melone MA, Cipollaro M, Peluso G, Galderisi U. Changes in autophagy, proteasome activity and metabolism to determine a specific signature for acute and chronic senescent mesenchymal stromal cells. Oncotarget. 2015;6(37):39457–68.PubMedPubMed CentralGoogle Scholar
- Ohdaira H, Sekiguchi M, Miyata K, Yoshida K. MicroRNA-494 suppresses cell proliferation and induces senescence in A549 lung cancer cells. Cell Prolif. 2012;45(1):32–8.View ArticlePubMedGoogle Scholar
- Xia Z, Dickens M, Raingeaud J, Davis RJ, Greenberg ME. Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis. Science. 1995;270(5240):1326–31.View ArticlePubMedGoogle Scholar
- Chen J, Fujii K, Zhang L, Roberts T, Fu H. Raf-1 promotes cell survival by antagonizing apoptosis signal-regulating kinase 1 through a MEK-ERK independent mechanism. Proc Natl Acad Sci USA. 2001;98(14):7783–8.View ArticlePubMedGoogle Scholar
- Saiki S, Sasazawa Y, Imamichi Y, Kawajiri S, Fujimaki T, Tanida I, Kobayashi H, Sato F, Sato S, Ishikawa K, et al. Caffeine induces apoptosis by enhancement of autophagy via PI3K/Akt/mTOR/p70S6K inhibition. Autophagy. 2011;7(2):176–87.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhong L, Shu W, Dai W, Gao B, Xiong S. Reactive oxygen species-mediated c-Jun NH2-terminal kinase activation contributes to hepatitis B virus X protein-induced autophagy via regulation of the Beclin-1/Bcl-2 interaction. J Virol. 2017;91(15):e00001–17.View ArticlePubMedPubMed CentralGoogle Scholar
- Radoshevich L, Murrow L, Chen N, Fernandez E, Roy S, Fung C, Debnath J. ATG12 conjugation to ATG3 regulates mitochondrial homeostasis and cell death. Cell. 2010;142(4):590–600.View ArticlePubMedPubMed CentralGoogle Scholar